A functional insulator screen identifies NURF and dREAM components to be required for enhancer-blocking.
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Buxa, Melanie K
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AbstractChromatin insulators of higher eukaryotes functionally divide the genome into active and inactive domains. Furthermore, insulators regulate enhancer/promoter communication, which is evident from the Drosophila bithorax locus in which a multitude of regulatory elements control segment specific gene activity. Centrosomal protein 190 (CP190) is targeted to insulators by CTCF or other insulator DNA-binding factors. Chromatin analyses revealed that insulators are characterized by open and nucleosome depleted regions. Here, we wanted to identify chromatin modification and remodelling factors required for an enhancer blocking function. We used the well-studied Fab-8 insulator of the bithorax locus to apply a genome-wide RNAi screen for factors that contribute to the enhancer blocking function of CTCF and CP190. Among 78 genes required for optimal Fab-8 mediated enhancer blocking, all four components of the NURF complex as well as several subunits of the dREAM complex were most evident. Mass spectrometric analyses of CTCF or CP190 bound proteins as well as immune precipitation confirmed NURF and dREAM binding. Both co-localise with most CP190 binding sites in the genome and chromatin immune precipitation showed that CP190 recruits NURF and dREAM. Nucleosome occupancy and histone H3 binding analyses revealed that CP190 mediated NURF binding results in nucleosomal depletion at CP190 binding sites. Thus, we conclude that CP190 binding to CTCF or to other DNA binding insulator factors mediates recruitment of NURF and dREAM. Furthermore, the enhancer blocking function of insulators is associated with nucleosomal depletion and requires NURF and dREAM.
CitationA functional insulator screen identifies NURF and dREAM components to be required for enhancer-blocking. 2014, 9 (9):e107765 PLoS ONE
AffiliationHelmholtz Centre for ifection research, Innhoffenstr. 7, D38124 Braunschweig, Germany.
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- The chromosomal association/dissociation of the chromatin insulator protein Cp190 of Drosophila melanogaster is mediated by the BTB/POZ domain and two acidic regions.
- Authors: Oliver D, Sheehan B, South H, Akbari O, Pai CY
- Issue date: 2010 Dec 31
- Active promoters and insulators are marked by the centrosomal protein 190.
- Authors: Bartkuhn M, Straub T, Herold M, Herrmann M, Rathke C, Saumweber H, Gilfillan GD, Becker PB, Renkawitz R
- Issue date: 2009 Apr 8
- Functional sub-division of the Drosophila genome via chromatin looping: the emerging importance of CP190.
- Authors: Ahanger SH, Shouche YS, Mishra RK
- Issue date: 2013 Mar-Apr
- Genome-Wide Mapping Targets of the Metazoan Chromatin Remodeling Factor NURF Reveals Nucleosome Remodeling at Enhancers, Core Promoters and Gene Insulators.
- Authors: Kwon SY, Grisan V, Jang B, Herbert J, Badenhorst P
- Issue date: 2016 Apr
- Coordinated control of dCTCF and gypsy chromatin insulators in Drosophila.
- Authors: Gerasimova TI, Lei EP, Bushey AM, Corces VG
- Issue date: 2007 Dec 14