Identification of the alternative sigma factor SigX regulon and its implications for Pseudomonas aeruginosa pathogenicity.
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Authors
Blanka, AndreaSchulz, Sebastian
Eckweiler, Denitsa
Franke, Raimo
Bielecka, Agata
Nicolai, Tanja
Casilag, Fiordiligie
Düvel, Juliane
Abraham, Wolf-Rainer

Kaever, Volkhard
Häussler, Susanne

Issue Date
2014-01
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Show full item recordAbstract
Pseudomonas aeruginosa is distinguished by its broad metabolic diversity and its remarkable capability for adaptation, which relies on a large collection of transcriptional regulators and alternative sigma (σ) factors. The largest group of alternative σ factors is that of the extracytoplasmic function (ECF) σ factors, which control key transduction pathways for maintenance of envelope homeostasis in response to external stress and cell growth. In addition, there are specific roles of alternative σ factors in regulating the expression of virulence and virulence-associated genes. Here, we analyzed a deletion mutant of the ECF σ factor SigX and applied mRNA profiling to define the SigX-dependent regulon in P. aeruginosa in response to low-osmolarity-medium conditions. Furthermore, the combination of transcriptional data with chromatin immunoprecipitation (ChIP) followed by high-throughput sequencing (ChIP-seq) led to the identification of the DNA binding motif of SigX. Genome-wide mapping of SigX-binding regions revealed enrichment of downstream genes involved in fatty acid biosynthesis, type III secretion, swarming and cyclic di-GMP (c-di-GMP) signaling. In accordance, a sigX deletion mutant exhibited altered fatty acid composition of the cell membrane, reduced cytotoxicity, impaired swarming activity, elevated c-di-GMP levels, and increased biofilm formation. In conclusion, a combination of ChIP-seq with transcriptional profiling and bioinformatic approaches to define consensus DNA binding sequences proved to be effective for the elucidation of the regulon of the alternative σ factor SigX, revealing its role in complex virulence-associated phenotypes in P. aeruginosa.Citation
Identification of the alternative sigma factor SigX regulon and its implications for Pseudomonas aeruginosa pathogenicity. 2014, 196 (2):345-56 J. Bacteriol.Affiliation
Helmholtz Centre of infection research; Inhoffenstr. 7; D38124 Braunschweig; Germany.Journal
Journal of bacteriologyPubMed ID
24187091Type
ArticleLanguage
enISSN
1098-5530ae974a485f413a2113503eed53cd6c53
10.1128/JB.01034-13
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