The EHEC-host interactome reveals novel targets for the translocated intimin receptor.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Schmidt, M Alexander
MetadataShow full item record
AbstractEnterohemorrhagic E. coli (EHEC) manipulate their human host through at least 39 effector proteins which hijack host processes through direct protein-protein interactions (PPIs). To identify their protein targets in the host cells, we performed yeast two-hybrid screens, allowing us to find 48 high-confidence protein-protein interactions between 15 EHEC effectors and 47 human host proteins. In comparison to other bacteria and viruses we found that EHEC effectors bind more frequently to hub proteins as well as to proteins that participate in a higher number of protein complexes. The data set includes six new interactions that involve the translocated intimin receptor (TIR), namely HPCAL1, HPCAL4, NCALD, ARRB1, PDE6D, and STK16. We compared these TIR interactions in EHEC and enteropathogenic E. coli (EPEC) and found that five interactions were conserved. Notably, the conserved interactions included those of serine/threonine kinase 16 (STK16), hippocalcin-like 1 (HPCAL1) as well as neurocalcin-delta (NCALD). These proteins co-localize with the infection sites of EPEC. Furthermore, our results suggest putative functions of poorly characterized effectors (EspJ, EspY1). In particular, we observed that EspJ is connected to the microtubule system while EspY1 appears to be involved in apoptosis/cell cycle regulation.
CitationThe EHEC-host interactome reveals novel targets for the translocated intimin receptor. 2014, 4:7531 Sci Rep
AffiliationHelmholtz Centre for infection research; Inhooffenstr. 7; D-38124 Braunschweig; Germany.
The following license files are associated with this item:
- Characterization of the binding surface of the translocated intimin receptor, an essential protein for EPEC and EHEC cell adhesion.
- Authors: Ross NT, Miller BL
- Issue date: 2007 Dec
- Crystal structure of EHEC intimin: insights into the complementarity between EPEC and EHEC.
- Authors: Yi Y, Ma Y, Gao F, Mao X, Peng H, Feng Y, Fan Z, Wang G, Guo G, Yan J, Zeng H, Zou Q, Gao GF
- Issue date: 2010 Dec 16
- Nck adaptors, besides promoting N-WASP mediated actin-nucleation activity at pedestals, influence the cellular levels of enteropathogenic Escherichia coli Tir effector.
- Authors: Nieto-Pelegrin E, Kenny B, Martinez-Quiles N
- Issue date: 2014
- Actin pedestal formation by enterohemorrhagic Escherichia coli enhances bacterial host cell attachment and concomitant type III translocation.
- Authors: Battle SE, Brady MJ, Vanaja SK, Leong JM, Hecht GA
- Issue date: 2014 Sep
- Dual infection system identifies a crucial role for PKA-mediated serine phosphorylation of the EPEC-Tir-injected effector protein in regulating Rac1 function.
- Authors: Brandt S, Kenny B, Rohde M, Martinez-Quiles N, Backert S
- Issue date: 2009 Aug