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dc.contributor.authorMayer, Christian T
dc.contributor.authorLahl, Katharina
dc.contributor.authorMilanez-Almeida, Pedro
dc.contributor.authorWatts, Deepika
dc.contributor.authorDittmer, Ulf
dc.contributor.authorFyhrquist, Nanna
dc.contributor.authorHuehn, Jochen
dc.contributor.authorKopf, Manfred
dc.contributor.authorKretschmer, Karsten
dc.contributor.authorRouse, Barry
dc.contributor.authorSparwasser, Tim
dc.date.accessioned2015-01-19T15:57:32Zen
dc.date.available2015-01-19T15:57:32Zen
dc.date.issued2014-11en
dc.identifier.citationAdvantages of Foxp3(+) regulatory T cell depletion using DEREG mice. 2014, 2 (3):162-5 Immun Inflamm Disen
dc.identifier.issn2050-4527en
dc.identifier.pmid25505550en
dc.identifier.doi10.1002/iid3.33en
dc.identifier.urihttp://hdl.handle.net/10033/338558en
dc.description.abstractSeveral mechanisms enable immunological self-tolerance. Regulatory T cells (Tregs) are a specialized T cell subset that prevents autoimmunity and excessive immune responses, but can also mediate detrimental tolerance to tumors and pathogens in a Foxp3-dependent manner. Genetic tools exploiting the foxp3 locus including bacterial artificial chromosome (BAC)-transgenic DEREG mice have provided essential information on Treg biology and the potential therapeutic modulation of tolerance. In DEREG mice, Foxp3(+) Tregs selectively express eGFP and diphtheria toxin (DT) receptor, allowing for the specific depletion of Tregs through DT administration. We here provide a detailed overview about important considerations such as DT toxicity, which affects any mouse strain treated with DT, and Treg rebound after depletion. Additionally, we point out the specific advantages of BAC-transgenic DEREG mice including their suitability to study organ-specific autoimmunity such as type I diabetes. Moreover, we discuss recent insights into the role of Tregs in viral infections. In summary, DEREG mice are an important tool to study Treg-mediated tolerance and its therapeutic circumvention.
dc.language.isoenen
dc.titleAdvantages of Foxp3(+) regulatory T cell depletion using DEREG mice.en
dc.typeArticleen
dc.identifier.journalImmunity, inflammation and diseaseen
refterms.dateFOA2018-06-13T21:21:31Z
html.description.abstractSeveral mechanisms enable immunological self-tolerance. Regulatory T cells (Tregs) are a specialized T cell subset that prevents autoimmunity and excessive immune responses, but can also mediate detrimental tolerance to tumors and pathogens in a Foxp3-dependent manner. Genetic tools exploiting the foxp3 locus including bacterial artificial chromosome (BAC)-transgenic DEREG mice have provided essential information on Treg biology and the potential therapeutic modulation of tolerance. In DEREG mice, Foxp3(+) Tregs selectively express eGFP and diphtheria toxin (DT) receptor, allowing for the specific depletion of Tregs through DT administration. We here provide a detailed overview about important considerations such as DT toxicity, which affects any mouse strain treated with DT, and Treg rebound after depletion. Additionally, we point out the specific advantages of BAC-transgenic DEREG mice including their suitability to study organ-specific autoimmunity such as type I diabetes. Moreover, we discuss recent insights into the role of Tregs in viral infections. In summary, DEREG mice are an important tool to study Treg-mediated tolerance and its therapeutic circumvention.


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