Optimized cryopreservation of mixed microbial communities for conserved functionality and diversity.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Courtens, Emilie N P
Pieper, Dietmar H
Vlaeminck, Siegfried E
Van de Wiele, Tom
MetadataShow full item record
AbstractThe use of mixed microbial communities (microbiomes) for biotechnological applications has steadily increased over the past decades. However, these microbiomes are not readily available from public culture collections, hampering their potential for widespread use. The main reason for this lack of availability is the lack of an effective cryopreservation protocol. Due to this critical need, we evaluated the functionality as well as the community structure of three different types of microbiomes before and after cryopreservation with two cryoprotective agents (CPA). Microbiomes were selected based upon relevance towards applications: (1) a methanotrophic co-culture (MOB), with potential for mitigation of greenhouse gas emissions, environmental pollutants removal and bioplastics production; (2) an oxygen limited autotrophic nitrification/denitrification (OLAND) biofilm, with enhanced economic and ecological benefits for wastewater treatment, and (3) fecal material from a human donor, with potential applications for fecal transplants and pre/probiotics research. After three months of cryopreservation at -80 °C, we found that metabolic activity, in terms of the specific activity recovery of MOB, aerobic ammonium oxidizing bacteria (AerAOB) and anaerobic AOB (AnAOB, anammox) in the OLAND mixed culture, resumes sooner when one of our selected CPA [dimethyl sulfoxide (DMSO) and DMSO plus trehalose and tryptic soy broth (DMSO+TT)] was added. However, the activity of the fecal community was not influenced by the CPA addition, although the preservation of the community structure (as determined by 16S rRNA gene sequencing) was enhanced by addition of CPA. In summary, we have evaluated a cryopreservation protocol that succeeded in preserving both community structure and functionality of value-added microbiomes. This will allow individual laboratories and culture collections to boost the use of microbiomes in biotechnological applications.
CitationOptimized cryopreservation of mixed microbial communities for conserved functionality and diversity. 2014, 9 (6):e99517 PLoS ONE
The following license files are associated with this item:
- Cryopreservation and fast recovery of enriched syngas-converting microbial communities.
- Authors: Grimalt-Alemany A, Łężyk M, Asimakopoulos K, Skiadas IV, Gavala HN
- Issue date: 2020 Jun 15
- A generally applicable cryopreservation method for nitrite-oxidizing bacteria.
- Authors: Vekeman B, Hoefman S, De Vos P, Spieck E, Heylen K
- Issue date: 2013 Dec
- Long-chain acylhomoserine lactones increase the anoxic ammonium oxidation rate in an OLAND biofilm.
- Authors: De Clippeleir H, Defoirdt T, Vanhaecke L, Vlaeminck SE, Carballa M, Verstraete W, Boon N
- Issue date: 2011 May
- Aggregate size and architecture determine microbial activity balance for one-stage partial nitritation and anammox.
- Authors: Vlaeminck SE, Terada A, Smets BF, De Clippeleir H, Schaubroeck T, Bolca S, Demeestere L, Mast J, Boon N, Carballa M, Verstraete W
- Issue date: 2010 Feb
- Efficient cryopreservation protocol enables accessibility of a broad range of ammonia-oxidizing bacteria for the scientific community.
- Authors: Hoefman S, Pommerening-Röser A, Samyn E, De Vos P, Heylen K
- Issue date: 2013 May