Influence of PhoP and intra-species variations on virulence of Yersinia pseudotuberculosis during the natural oral infection route.
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AbstractThe two-component regulatory system PhoP/PhoQ has been shown to (i) control expression of virulence-associated traits, (ii) confer survival and growth within macrophages and (iii) play a role in Yersinia infections. However, the influence of PhoP on virulence varied greatly between different murine models of infection and its role in natural oral infections with frequently used representative isolates of Y. pseudotuberculosis was unknown. To address this issue, we constructed an isogenic set of phoP+ and phoP- variants of strain IP32953 and YPIII and analyzed the impact of PhoP using in vitro functionality experiments and a murine oral infection model, whereby we tested for bacterial dissemination and influence on the host immune response. Our results revealed that PhoP has a low impact on virulence, lymphatic and systemic organ colonization, and on immune response modulation by IP32953 and YPIII, indicating that PhoP is not absolutely essential for oral infections but may be involved in fine-tuning the outcome. Our work further revealed certain strain-specific differences in virulence properties, which do not strongly rely on the function of PhoP, but affect tissue colonization, dissemination and/or persistence of the bacteria. Highlighted intra-species variations may provide a potential means to rapidly adjust to environmental changes inside and outside of the host.
CitationInfluence of PhoP and intra-species variations on virulence of Yersinia pseudotuberculosis during the natural oral infection route. 2014, 9 (7):e103541 PLoS ONE
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- The role of the phoPQ operon in the pathogenesis of the fully virulent CO92 strain of Yersinia pestis and the IP32953 strain of Yersinia pseudotuberculosis.
- Authors: Bozue J, Mou S, Moody KL, Cote CK, Trevino S, Fritz D, Worsham P
- Issue date: 2011 Jun
- The response regulator PhoP of Yersinia pseudotuberculosis is important for replication in macrophages and for virulence.
- Authors: Grabenstein JP, Marceau M, Pujol C, Simonet M, Bliska JB
- Issue date: 2004 Sep
- Th1-type immune response to infection by pYV-cured phoP-phoQ null mutant of Yersinia pseudotuberculosis is defective in mouse model.
- Authors: Kumar S, Balakrishna K, Agarwal GS, Merwyn S, Rai GP, Batra HV, Sardesai AA, Gowrishankar J
- Issue date: 2009 Jan
- Intranasal inoculation of mice with Yersinia pseudotuberculosis causes a lethal lung infection that is dependent on Yersinia outer proteins and PhoP.
- Authors: Fisher ML, Castillo C, Mecsas J
- Issue date: 2007 Jan
- A direct link between the global regulator PhoP and the Csr regulon in Y. pseudotuberculosis through the small regulatory RNA CsrC.
- Authors: Nuss AM, Schuster F, Kathrin Heroven A, Heine W, Pisano F, Dersch P
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