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dc.contributor.authorPisano, Fabio
dc.contributor.authorHeine, Wiebke
dc.contributor.authorRosenheinrich, Maik
dc.contributor.authorSchweer, Janina
dc.contributor.authorNuss, Aaron M
dc.contributor.authorDersch, Petra
dc.date.accessioned2015-01-22T12:24:14Zen
dc.date.available2015-01-22T12:24:14Zen
dc.date.issued2014en
dc.identifier.citationInfluence of PhoP and intra-species variations on virulence of Yersinia pseudotuberculosis during the natural oral infection route. 2014, 9 (7):e103541 PLoS ONEen
dc.identifier.issn1932-6203en
dc.identifier.pmid25075520en
dc.identifier.doi10.1371/journal.pone.0103541en
dc.identifier.urihttp://hdl.handle.net/10033/338665en
dc.description.abstractThe two-component regulatory system PhoP/PhoQ has been shown to (i) control expression of virulence-associated traits, (ii) confer survival and growth within macrophages and (iii) play a role in Yersinia infections. However, the influence of PhoP on virulence varied greatly between different murine models of infection and its role in natural oral infections with frequently used representative isolates of Y. pseudotuberculosis was unknown. To address this issue, we constructed an isogenic set of phoP+ and phoP- variants of strain IP32953 and YPIII and analyzed the impact of PhoP using in vitro functionality experiments and a murine oral infection model, whereby we tested for bacterial dissemination and influence on the host immune response. Our results revealed that PhoP has a low impact on virulence, lymphatic and systemic organ colonization, and on immune response modulation by IP32953 and YPIII, indicating that PhoP is not absolutely essential for oral infections but may be involved in fine-tuning the outcome. Our work further revealed certain strain-specific differences in virulence properties, which do not strongly rely on the function of PhoP, but affect tissue colonization, dissemination and/or persistence of the bacteria. Highlighted intra-species variations may provide a potential means to rapidly adjust to environmental changes inside and outside of the host.
dc.language.isoenen
dc.titleInfluence of PhoP and intra-species variations on virulence of Yersinia pseudotuberculosis during the natural oral infection route.en
dc.typeArticleen
dc.identifier.journalPloS oneen
refterms.dateFOA2018-06-13T19:56:29Z
html.description.abstractThe two-component regulatory system PhoP/PhoQ has been shown to (i) control expression of virulence-associated traits, (ii) confer survival and growth within macrophages and (iii) play a role in Yersinia infections. However, the influence of PhoP on virulence varied greatly between different murine models of infection and its role in natural oral infections with frequently used representative isolates of Y. pseudotuberculosis was unknown. To address this issue, we constructed an isogenic set of phoP+ and phoP- variants of strain IP32953 and YPIII and analyzed the impact of PhoP using in vitro functionality experiments and a murine oral infection model, whereby we tested for bacterial dissemination and influence on the host immune response. Our results revealed that PhoP has a low impact on virulence, lymphatic and systemic organ colonization, and on immune response modulation by IP32953 and YPIII, indicating that PhoP is not absolutely essential for oral infections but may be involved in fine-tuning the outcome. Our work further revealed certain strain-specific differences in virulence properties, which do not strongly rely on the function of PhoP, but affect tissue colonization, dissemination and/or persistence of the bacteria. Highlighted intra-species variations may provide a potential means to rapidly adjust to environmental changes inside and outside of the host.


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