Efficient nanoparticle-mediated needle-free transcutaneous vaccination via hair follicles requires adjuvantation.

Mittal, Ankit; Schulze, Kai; Ebensen, Thomas; Weißmann, Sebastian; Hansen, Steffi; Lehr, Claus-Michael; Guzmán, Carlos A

dc.contributor.author	Mittal, Ankit
dc.contributor.author	Schulze, Kai
dc.contributor.author	Ebensen, Thomas
dc.contributor.author	Weißmann, Sebastian
dc.contributor.author	Hansen, Steffi
dc.contributor.author	Lehr, Claus-Michael
dc.contributor.author	Guzmán, Carlos A
dc.date.accessioned	2015-02-11T15:49:31Z	en
dc.date.available	2015-02-11T15:49:31Z	en
dc.date.issued	2015-01	en
dc.identifier.citation	Efficient nanoparticle-mediated needle-free transcutaneous vaccination via hair follicles requires adjuvantation. 2015, 11 (1):147-54 Nanomedicine	en
dc.identifier.issn	1549-9642	en
dc.identifier.pmid	25200611	en
dc.identifier.doi	10.1016/j.nano.2014.08.009	en
dc.identifier.uri	http://hdl.handle.net/10033/344377	en
dc.description.abstract	Trans-follicular (TF) vaccination has recently been studied as a unique route for non-invasive transcutaneous vaccination. The present study aims to extensively characterize the immune responses triggered by TF vaccination using ovalbumin loaded chitosan-PLGA (poly lactic-co-glycolic acid) nanoparticles without skin pre-treatment to preserve skin integrity. The impact of formulation composition i.e. antigenic solution or antigen-loaded nanoparticles with or without adjuvant [bis-(3',5')-cyclic dimeric adenosine monophosphate] on immune response quality following TF immunization was analyzed and compared with immune responses obtained after tape stripping the skin. The results presented in this study confirm the ability of nanoparticle based vaccine formulations to deliver antigen across the intact skin via the follicular route, but at the same time demonstrate the necessity to include adjuvants to generate efficient antigen-specific humoral and cellular immune responses.
dc.language.iso	en	en
dc.title	Efficient nanoparticle-mediated needle-free transcutaneous vaccination via hair follicles requires adjuvantation.	en
dc.type	Article	en
dc.contributor.department	Helmholtz Centre for Infection Research (HZI), Braunschweig, Germany.	en
dc.identifier.journal	Nanomedicine : nanotechnology, biology, and medicine	en
refterms.dateFOA	2018-06-12T22:33:44Z
html.description.abstract	Trans-follicular (TF) vaccination has recently been studied as a unique route for non-invasive transcutaneous vaccination. The present study aims to extensively characterize the immune responses triggered by TF vaccination using ovalbumin loaded chitosan-PLGA (poly lactic-co-glycolic acid) nanoparticles without skin pre-treatment to preserve skin integrity. The impact of formulation composition i.e. antigenic solution or antigen-loaded nanoparticles with or without adjuvant [bis-(3',5')-cyclic dimeric adenosine monophosphate] on immune response quality following TF immunization was analyzed and compared with immune responses obtained after tape stripping the skin. The results presented in this study confirm the ability of nanoparticle based vaccine formulations to deliver antigen across the intact skin via the follicular route, but at the same time demonstrate the necessity to include adjuvants to generate efficient antigen-specific humoral and cellular immune responses.