Gulosibacter molinativorax ON4T molinate hydrolase, a novel cobalt-dependent amidohydrolase.
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Pieper, Dietmar H
Nunes, Olga C
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AbstractA new pathway of molinate mineralization has recently been described. Among the five members of the mixed culture able to promote such a process, Gulosibacter molinativorax ON4(T) has been observed to promote the initial breakdown of the herbicide into ethanethiol and azepane-1-carboxylate. In the current study, the gene encoding the enzyme responsible for molinate hydrolysis was identified and heterologously expressed, and the resultant active protein was purified and characterized. Nucleotide sequence analysis revealed that the gene encodes a 465-amino-acid protein of the metal-dependent hydrolase A subfamily of the amidohydrolase superfamily with a predicted molecular mass of 50.9 kDa. Molinate hydrolase shares the highest amino acid sequence identity (48 to 50%) with phenylurea hydrolases of Arthrobacter globiformis and Mycobacterium brisbanense. However, in contrast to previously described members of the metal-dependent hydrolase A subfamily, molinate hydrolase contains cobalt as the only active-site metal.
CitationGulosibacter molinativorax ON4T molinate hydrolase, a novel cobalt-dependent amidohydrolase. 2011, 193 (20):5810-6 J. Bacteriol.
JournalJournal of bacteriology
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- New insights into a bacterial metabolic and detoxifying association responsible for the mineralization of the thiocarbamate herbicide molinate.
- Authors: Barreiros L, Fernandes A, Ferreira ACS, Pereira H, Bastos MMSM, Manaia CM, Nunes OC
- Issue date: 2008 Apr
- In silico prediction of the enzymes involved in the degradation of the herbicide molinate by Gulosibacter molinativorax ON4<sup>T</sup>.
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- Microencapsulation of Gulosibacter molinativorax ON4<sup>T</sup> cells by a spray-drying process using different biopolymers.
- Authors: Estevinho BN, Lopes AR, Sousa V, Rocha F, Nunes OC
- Issue date: 2017 Sep 15
- Structure-guided engineering of molinate hydrolase for the degradation of thiocarbamate pesticides.
- Authors: Leite JP, Duarte M, Paiva AM, Ferreira-da-Silva F, Matias PM, Nunes OC, Gales L
- Issue date: 2015
- Gulosibacter molinativorax gen. nov., sp. nov., a molinate-degrading bacterium, and classification of 'Brevibacterium helvolum' DSM 20419 as Pseudoclavibacter helvolus gen. nov., sp. nov.
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- Issue date: 2004 May