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dc.contributor.authorSchulz, Sebastian
dc.contributor.authorEckweiler, Denitsa
dc.contributor.authorBielecka, Agata
dc.contributor.authorNicolai, Tanja
dc.contributor.authorFranke, Raimo
dc.contributor.authorDötsch, Andreas
dc.contributor.authorHornischer, Klaus
dc.contributor.authorBruchmann, Sebastian
dc.contributor.authorDüvel, Juliane
dc.contributor.authorHäussler, Susanne
dc.date.accessioned2015-03-18T14:57:33Zen
dc.date.available2015-03-18T14:57:33Zen
dc.date.issued2015-03en
dc.identifier.citationElucidation of Sigma Factor-Associated Networks in Pseudomonas aeruginosa Reveals a Modular Architecture with Limited and Function-Specific Crosstalk. 2015, 11 (3):e1004744 PLoS Pathog.en
dc.identifier.issn1553-7374en
dc.identifier.pmid25780925en
dc.identifier.doi10.1371/journal.ppat.1004744en
dc.identifier.urihttp://hdl.handle.net/10033/346846en
dc.description.abstractSigma factors are essential global regulators of transcription initiation in bacteria which confer promoter recognition specificity to the RNA polymerase core enzyme. They provide effective mechanisms for simultaneously regulating expression of large numbers of genes in response to challenging conditions, and their presence has been linked to bacterial virulence and pathogenicity. In this study, we constructed nine his-tagged sigma factor expressing and/or deletion mutant strains in the opportunistic pathogen Pseudomonas aeruginosa. To uncover the direct and indirect sigma factor regulons, we performed mRNA profiling, as well as chromatin immunoprecipitation coupled to high-throughput sequencing. We furthermore elucidated the de novo binding motif of each sigma factor, and validated the RNA- and ChIP-seq results by global motif searches in the proximity of transcriptional start sites (TSS). Our integrated approach revealed a highly modular network architecture which is composed of insulated functional sigma factor modules. Analysis of the interconnectivity of the various sigma factor networks uncovered a limited, but highly function-specific, crosstalk which orchestrates complex cellular processes. Our data indicate that the modular structure of sigma factor networks enables P. aeruginosa to function adequately in its environment and at the same time is exploited to build up higher-level functions by specific interconnections that are dominated by a participation of RpoN.
dc.languageENGen
dc.titleElucidation of Sigma Factor-Associated Networks in Pseudomonas aeruginosa Reveals a Modular Architecture with Limited and Function-Specific Crosstalk.en
dc.typeArticleen
dc.contributor.departmentInstitute for Molecular Bacteriology, TWINCORE GmbH.en
dc.identifier.journalPLoS pathogensen
refterms.dateFOA2018-06-13T00:15:33Z
html.description.abstractSigma factors are essential global regulators of transcription initiation in bacteria which confer promoter recognition specificity to the RNA polymerase core enzyme. They provide effective mechanisms for simultaneously regulating expression of large numbers of genes in response to challenging conditions, and their presence has been linked to bacterial virulence and pathogenicity. In this study, we constructed nine his-tagged sigma factor expressing and/or deletion mutant strains in the opportunistic pathogen Pseudomonas aeruginosa. To uncover the direct and indirect sigma factor regulons, we performed mRNA profiling, as well as chromatin immunoprecipitation coupled to high-throughput sequencing. We furthermore elucidated the de novo binding motif of each sigma factor, and validated the RNA- and ChIP-seq results by global motif searches in the proximity of transcriptional start sites (TSS). Our integrated approach revealed a highly modular network architecture which is composed of insulated functional sigma factor modules. Analysis of the interconnectivity of the various sigma factor networks uncovered a limited, but highly function-specific, crosstalk which orchestrates complex cellular processes. Our data indicate that the modular structure of sigma factor networks enables P. aeruginosa to function adequately in its environment and at the same time is exploited to build up higher-level functions by specific interconnections that are dominated by a participation of RpoN.


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