Differentially oriented populations of actin filaments generated in lamellipodia collaborate in pushing and pausing at the cell front.
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Issue Date
2008-03
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Show full item recordAbstract
Eukaryotic cells advance in phases of protrusion, pause and withdrawal. Protrusion occurs in lamellipodia, which are composed of diagonal networks of actin filaments, and withdrawal terminates with the formation of actin bundles parallel to the cell edge. Using correlated live-cell imaging and electron microscopy, we have shown that actin filaments in protruding lamellipodia subtend angles from 15-90 degrees to the front, and that transitions from protrusion to pause are associated with a proportional increase in filaments oriented more parallel to the cell edge. Microspike bundles of actin filaments also showed a wide angular distribution and correspondingly variable bilateral polymerization rates along the cell front. We propose that the angular shift of filaments in lamellipodia serves in adapting to slower protrusion rates while maintaining the filament densities required for structural support; further, we suggest that single filaments and microspike bundles contribute to the construction of the lamella behind and to the formation of the cell edge when protrusion ceases. Our findings provide an explanation for the variable turnover dynamics of actin filaments in lamellipodia observed by fluorescence speckle microscopy and are inconsistent with a current model of lamellipodia structure that features actin filaments branching at 70 degrees in a dendritic array.Citation
Differentially oriented populations of actin filaments generated in lamellipodia collaborate in pushing and pausing at the cell front. 2008, 10 (3):306-13 Nat. Cell Biol.Affiliation
Institute of Molecular Biotechnology, Austrian Academy of Sciences, Dr. Bohr-Gasse 3, 1030, Vienna, Austria.Journal
Nature cell biologyDOI
10.1038/ncb1692PubMed ID
18278037Type
ArticleLanguage
enISSN
1476-4679ae974a485f413a2113503eed53cd6c53
10.1038/ncb1692
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