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    The 'pH optimum anomaly' of intracellular enzymes of Ferroplasma acidiphilum.

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    Authors
    Golyshina, Olga V
    Golyshin, Peter N
    Timmis, Kenneth N
    Ferrer, Manuel
    Issue Date
    2006-03
    
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    Abstract
    A wide range of microorganisms, the so-called acidophiles, inhabit acidic environments and grow optimally at pH values between 0 and 3. The intracellular pH of these organisms is, however, close to neutrality or slightly acidic. It is to be expected that enzymatic activities dedicated to extracellular functions would be adapted to the prevailing low pH of the environment (0-3), whereas intracellular enzymes would be optimally active at the near-neutral pH of the cytoplasm (4.6-7.0). The genes of several intracellular or cell-bound enzymes, a carboxylesterase and three alpha-glucosidases, from Ferroplasma acidiphilum, a cell wall-lacking acidophilic archaeon with a growth optimum at pH 1.7, were cloned and expressed in Escherichia coli, and their products purified and characterized. The Ferroplasmaalpha-glucosidases exhibited no sequence similarity to known glycosyl hydrolases. All enzymes functioned and were stable in vitro in the pH range 1.7-4.0, and had pH optima much lower than the mean intracellular pH of 5.6. This 'pH optimum anomaly' suggests the existence of yet-undetected cellular compartmentalization providing cytoplasmic pH patchiness and low pH environments for the enzymes we have analysed.
    Citation
    The 'pH optimum anomaly' of intracellular enzymes of Ferroplasma acidiphilum. 2006, 8 (3):416-25 Environ. Microbiol.
    Affiliation
    Division of Microbiology, GBF--German Research Centre for Biotechnology, Braunschweig, Germany.
    Journal
    Environmental microbiology
    URI
    http://hdl.handle.net/10033/50053
    DOI
    10.1111/j.1462-2920.2005.00907.x
    PubMed ID
    16478448
    Type
    Article
    Language
    en
    ISSN
    1462-2912
    ae974a485f413a2113503eed53cd6c53
    10.1111/j.1462-2920.2005.00907.x
    Scopus Count
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    Publications of RG Environmental Microbiology (UMW)

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