Constitutive production of c-di-GMP is associated with mutations in a variant of Pseudomonas aeruginosa with altered membrane composition.
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Authors
Blanka, AndreaDüvel, Juliane
Dötsch, Andreas
Klinkert, Birgit
Abraham, Wolf-Rainer

Kaever, Volkhard
Ritter, Christiane
Narberhaus, Franz
Häussler, Susanne
Issue Date
2015
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Show full item recordAbstract
Most bacteria can form multicellular communities called biofilms on biotic and abiotic surfaces. This multicellular response to surface contact correlates with an increased resistance to various adverse environmental conditions, including those encountered during infections of the human host and exposure to antimicrobial compounds. Biofilm formation occurs when freely swimming (planktonic) cells encounter a surface, which stimulates the chemosensory-like, surface-sensing system Wsp and leads to generation of the intracellular second messenger 3',5'-cyclic-di-guanosine monophosphate (c-di-GMP). We identified adaptive mutations in a clinical small colony variant (SCV) of Pseudomonas aeruginosa and correlated their presence with self-aggregating growth behavior and an enhanced capacity to form biofilms. We present evidence that a point mutation in the 5' untranslated region of the accBC gene cluster, which encodes components of an enzyme responsible for fatty acid biosynthesis, was responsible for a stabilized mRNA structure that resulted in reduced translational efficiency and an increase in the proportion of short-chain fatty acids in the plasma membrane. We propose a model in which these changes in P. aeruginosa serve as a signal for the Wsp system to constitutively produce increased amounts of c-di-GMP and thus play a role in the regulation of adhesion-stimulated bacterial responses.Citation
Constitutive production of c-di-GMP is associated with mutations in a variant of Pseudomonas aeruginosa with altered membrane composition. 2015, 8 (372):ra36 Sci SignalAffiliation
Institute for Molecular Bacteriology, TWINCORE,30625 Hannover, Germany.Journal
Science signalingPubMed ID
25872871Type
ArticleLanguage
enISSN
1937-9145ae974a485f413a2113503eed53cd6c53
10.1126/scisignal.2005943
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