Production and Properties of Xylan-Degrading Enzymes from Cellulomonas uda.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractXylan degradation and production of beta-xylanase and beta-xylosidase activities were studied in cultures of Cellulomonas uda grown on purified xylan from birchwood. beta-Xylanase activity was found to be associated with the cells, although in various degrees. The formation of beta-xylanase activity was induced by xylotriose and repressed by xylose. beta-Xylosidase activity was cell bound. Both constitutive and inducible beta-xylosidase activities were suggested. beta-Xylanase and beta-xylosidase activities were inhibited competitively by xylose. beta-Xylanase activity had a pronounced optimum pH of 5.8, whereas the optimum pH of beta-xylosidase activity ranged from 5.4 to 6.1. The major products of xylan degradation by a crude preparation of beta-xylanase activity, in decreasing order of amount, were xylobiose, xylotriose, xylose, and small amounts of xylotetraose. This pattern suggests that beta-xylanase activity secreted by C. uda is of the endosplitting type. Supernatants of cultures grown on cellulose showed not only beta-glucanase but also beta-xylanase activity. The latter could be attributed to an endo-1,4-beta-glucanase activity which had a low beta-xylanase activity.
CitationProduction and Properties of Xylan-Degrading Enzymes from Cellulomonas uda. 1986, 51 (4):746-52 Appl. Environ. Microbiol.
AffiliationGesellschaft fur Biotechnologische Forschung mbH, D-38124 Braunschweig, Germany.
The following license files are associated with this item:
- Modification of the carbohydrate composition of sulfite pulp by purified and characterized beta-xylanase and beta-xylosidase of Aureobasidium pullulans.
- Authors: Christov LP, Myburgh J, O'Neill FH, Van Tonder A, Prior BA
- Issue date: 1999 Mar-Apr
- Secretory expression and characterization of two hemicellulases, xylanase, and β-xylosidase, isolated from Bacillus subtilis M015.
- Authors: Banka AL, Guralp SA, Gulari E
- Issue date: 2014 Dec
- Degradation of xylan to D-xylose by recombinant Saccharomyces cerevisiae coexpressing the Aspergillus niger beta-xylosidase (xlnD) and the Trichoderma reesei xylanase II (xyn2) genes.
- Authors: La Grange DC, Pretorius IS, Claeyssens M, van Zyl WH
- Issue date: 2001 Dec
- Xylan-degrading enzymes of the yeast Cryptococcus albidus. Identification and cellular localization.
- Authors: Biely P, Vrsanská M, Krátký Z
- Issue date: 1980
- Production of xylanase by the ruminal anaerobic fungus Neocallimastix frontalis.
- Authors: Mountfort DO, Asher RA
- Issue date: 1989 Apr