Development of a unique epigenetic signature during in vivo Th17 differentiation.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Deyneko, Igor V
MetadataShow full item record
AbstractActivated naive CD4(+) T cells are highly plastic cells that can differentiate into various T helper (Th) cell fates characterized by the expression of effector cytokines like IFN-γ (Th1), IL-4 (Th2) or IL-17A (Th17). Although previous studies have demonstrated that epigenetic mechanisms including DNA demethylation can stabilize effector cytokine expression, a comprehensive analysis of the changes in the DNA methylation pattern during differentiation of naive T cells into Th cell subsets is lacking. Hence, we here performed a genome-wide methylome analysis of ex vivo isolated naive CD4(+) T cells, Th1 and Th17 cells. We could demonstrate that naive CD4(+) T cells share more demethylated regions with Th17 cells when compared to Th1 cells, and that overall Th17 cells display the highest number of demethylated regions, findings which are in line with the previously reported plasticity of Th17 cells. We could identify seven regions located in Il17a, Zfp362, Ccr6, Acsbg1, Dpp4, Rora and Dclk1 showing pronounced demethylation selectively in ex vivo isolated Th17 cells when compared to other ex vivo isolated Th cell subsets and in vitro generated Th17 cells, suggesting that this unique epigenetic signature allows identifying and functionally characterizing in vivo generated Th17 cells.
CitationDevelopment of a unique epigenetic signature during in vivo Th17 differentiation. 2015, 43 (3):1537-48 Nucleic Acids Res.
AffiliationHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
JournalNucleic acids research
The following license files are associated with this item:
- CD44 Reciprocally regulates the differentiation of encephalitogenic Th1/Th17 and Th2/regulatory T cells through epigenetic modulation involving DNA methylation of cytokine gene promoters, thereby controlling the development of experimental autoimmune encephalomyelitis.
- Authors: Guan H, Nagarkatti PS, Nagarkatti M
- Issue date: 2011 Jun 15
- Human Th1 and Th17 cells exhibit epigenetic stability at signature cytokine and transcription factor loci.
- Authors: Cohen CJ, Crome SQ, MacDonald KG, Dai EL, Mager DL, Levings MK
- Issue date: 2011 Dec 1
- Epigenetic changes at Il12rb2 and Tbx21 in relation to plasticity behavior of Th17 cells.
- Authors: Bending D, Newland S, Krejcí A, Phillips JM, Bray S, Cooke A
- Issue date: 2011 Mar 15
- Pharmacologic inhibition of RORγt regulates Th17 signature gene expression and suppresses cutaneous inflammation in vivo.
- Authors: Skepner J, Ramesh R, Trocha M, Schmidt D, Baloglu E, Lobera M, Carlson T, Hill J, Orband-Miller LA, Barnes A, Boudjelal M, Sundrud M, Ghosh S, Yang J
- Issue date: 2014 Mar 15
- Demethylation of the RORC2 and IL17A in human CD4+ T lymphocytes defines Th17 origin of nonclassic Th1 cells.
- Authors: Mazzoni A, Santarlasci V, Maggi L, Capone M, Rossi MC, Querci V, De Palma R, Chang HD, Thiel A, Cimaz R, Liotta F, Cosmi L, Maggi E, Radbruch A, Romagnani S, Dong J, Annunziato F
- Issue date: 2015 Apr 1