A Spatial Model of Insulin-Granule Dynamics in Pancreatic β-Cells.

Dehghany, Jaber; Hoboth, Peter; Ivanova, Anna; Mziaut, Hassan; Müller, Andreas; Kalaidzidis, Yannis; Solimena, Michele; Müller, A

dc.contributor.author	Dehghany, Jaber
dc.contributor.author	Hoboth, Peter
dc.contributor.author	Ivanova, Anna
dc.contributor.author	Mziaut, Hassan
dc.contributor.author	Müller, Andreas
dc.contributor.author	Kalaidzidis, Yannis
dc.contributor.author	Solimena, Michele
dc.contributor.author	Müller, A
dc.date.accessioned	2015-08-13T09:04:22Z	en
dc.date.available	2015-08-13T09:04:22Z	en
dc.date.issued	2015-08	en
dc.identifier.citation	A Spatial Model of Insulin-Granule Dynamics in Pancreatic β-Cells. 2015, 16 (8):797-813 Traffic	en
dc.identifier.issn	1600-0854	en
dc.identifier.pmid	25809669	en
dc.identifier.doi	10.1111/tra.12286	en
dc.identifier.uri	http://hdl.handle.net/10033/566265	en
dc.description.abstract	Insulin secretion from pancreatic β-cells in response to sudden glucose stimulation is biphasic. Prolonged secretion in vivo requires synthesis, delivery to the plasma membrane (PM) and exocytosis of insulin secretory granules (SGs). Here, we provide the first agent-based space-resolved model for SG dynamics in pancreatic β-cells. Using recent experimental data, we consider a single β-cell with identical SGs moving on a phenomenologically represented cytoskeleton network. A single exocytotic machinery mediates SG exocytosis on the PM. This novel model reproduces the measured spatial organization of SGs and insulin secretion patterns under different stimulation protocols. It proposes that the insulin potentiation effect and the rising second-phase secretion are mainly due to the increasing number of docking sites on the PM. Furthermore, it shows that 6 min after glucose stimulation, the 'newcomer' SGs are recruited from a region within less than 600 nm from the PM.
dc.language.iso	en	en
dc.title	A Spatial Model of Insulin-Granule Dynamics in Pancreatic β-Cells.	en
dc.type	Article	en
dc.contributor.department	Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.	en
dc.identifier.journal	Traffic (Copenhagen, Denmark)	en
refterms.dateFOA	2016-08-01T00:00:00Z
html.description.abstract	Insulin secretion from pancreatic β-cells in response to sudden glucose stimulation is biphasic. Prolonged secretion in vivo requires synthesis, delivery to the plasma membrane (PM) and exocytosis of insulin secretory granules (SGs). Here, we provide the first agent-based space-resolved model for SG dynamics in pancreatic β-cells. Using recent experimental data, we consider a single β-cell with identical SGs moving on a phenomenologically represented cytoskeleton network. A single exocytotic machinery mediates SG exocytosis on the PM. This novel model reproduces the measured spatial organization of SGs and insulin secretion patterns under different stimulation protocols. It proposes that the insulin potentiation effect and the rising second-phase secretion are mainly due to the increasing number of docking sites on the PM. Furthermore, it shows that 6 min after glucose stimulation, the 'newcomer' SGs are recruited from a region within less than 600 nm from the PM.