Biosynthesis of crocacin involves an unusual hydrolytic release domain showing similarity to condensation domains.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractThe crocacins are potent antifungal and cytotoxic natural compounds from myxobacteria of the genus Chondromyces. Although total synthesis approaches have been reported, the molecular and biochemical basis guiding the formation of the linear crocacin scaffold has remained unknown. Along with the identification and functional analysis of the crocacin biosynthetic gene cluster from Chondromyces crocatus Cm c5, we here present the identification and biochemical characterization of an unusual chain termination domain homologous to condensation domains responsible for hydrolytic release of the product from the assembly line. In particular, gene inactivation studies and in vitro experiments using the heterologously produced domain CroK-C2 confirm this surprising role giving rise to the linear carboxylic acid. Additionally, we determined the kinetic parameters of CroK-C2 by monitoring hydrolytic cleavage of the substrate mimic N-acetylcysteaminyl-crocacin B using an innovative high-performance liquid chromatography mass spectrometry-based assay.
CitationBiosynthesis of crocacin involves an unusual hydrolytic release domain showing similarity to condensation domains. 2014, 21 (7):855-65 Chem. Biol.
AffiliationHelmholtz Institute for Pharmaceutical Research Saarland,Saarbru¨ cken, Saarland 66123, Germany.
JournalChemistry & biology
The following license files are associated with this item:
- Molecular and biochemical studies of chondramide formation-highly cytotoxic natural products from Chondromyces crocatus Cm c5.
- Authors: Rachid S, Krug D, Kunze B, Kochems I, Scharfe M, Zabriskie TM, Blöcker H, Müller R
- Issue date: 2006 Jun
- Biosynthesis of thuggacins in myxobacteria: comparative cluster analysis reveals basis for natural product structural diversity.
- Authors: Buntin K, Irschik H, Weissman KJ, Luxenburger E, Blöcker H, Müller R
- Issue date: 2010 Apr 23
- Genome Analysis of the Fruiting Body-Forming Myxobacterium Chondromyces crocatus Reveals High Potential for Natural Product Biosynthesis.
- Authors: Zaburannyi N, Bunk B, Maier J, Overmann J, Müller R
- Issue date: 2016 Jan 15
- Crocacin, a new electron transport inhibitor from Chondromyces crocatus (myxobacteria). Production, isolation, physico-chemical and biological properties.
- Authors: Kunze B, Jansen R, Höfle G, Reichenbach H
- Issue date: 1994 Aug
- Crocadepsins-Depsipeptides from the Myxobacterium Chondromyces crocatus Found by a Genome Mining Approach.
- Authors: Surup F, Viehrig K, Rachid S, Plaza A, Maurer CK, Hartmann RW, Müller R
- Issue date: 2018 Jan 19