Extracellular milieu grossly alters pathogen-specific immune response of mammary epithelial cells.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractConsiderably divergent data have been published from attempts to model the E. coli vs. S. aureus specific immune reaction of the udder using primary cultures of bovine mammary epithelial cells from cows (pbMEC). Some groups reported a swift, strong and transient inflammatory response against challenges with E. coli and only a weak and retarded response against S. aureus, in agreement with the respective reaction of the udder. Others found almost the reverse. Presence or absence of fetal calf serum distinguished the experimental setting between both groups. We examined here if this causes the divergent reaction of the pbMEC towards both pathogen species. We challenged pbMEC with proteins from heat killed E. coli or S. aureus pathogens or purified TLR2 and TLR4 ligands. The stimuli were applied in normal growth medium with (SM10) or without (SM0) 10% fetal calf serum, or in the basal medium supplemented with 10 mg/ml milk proteins (SM Milk).
CitationExtracellular milieu grossly alters pathogen-specific immune response of mammary epithelial cells. 2015, 11:172 BMC Vet. Res.
AffiliationHelmholtz Center for Infection Research
JournalBMC veterinary research
The following license files are associated with this item:
- Bovine TLR2 and TLR4 properly transduce signals from Staphylococcus aureus and E. coli, but S. aureus fails to both activate NF-kappaB in mammary epithelial cells and to quickly induce TNFalpha and interleukin-8 (CXCL8) expression in the udder.
- Authors: Yang W, Zerbe H, Petzl W, Brunner RM, Günther J, Draing C, von Aulock S, Schuberth HJ, Seyfert HM
- Issue date: 2008 Mar
- Staphylococcus aureus and Escherichia coli elicit different innate immune responses from bovine mammary epithelial cells.
- Authors: Fu Y, Zhou E, Liu Z, Li F, Liang D, Liu B, Song X, Zhao F, Fen X, Li D, Cao Y, Zhang X, Zhang N, Yang Z
- Issue date: 2013 Oct 1
- NF-kappaB factors are essential, but not the switch, for pathogen-related induction of the bovine beta-defensin 5-encoding gene in mammary epithelial cells.
- Authors: Yang W, Molenaar A, Kurts-Ebert B, Seyfert HM
- Issue date: 2006 Feb
- Bacterial lipopolysaccharide induces increased expression of toll-like receptor (TLR) 4 and downstream TLR signaling molecules in bovine mammary epithelial cells.
- Authors: Ibeagha-Awemu EM, Lee JW, Ibeagha AE, Bannerman DD, Paape MJ, Zhao X
- Issue date: 2008 Mar-Apr
- Staphylococcus aureus induces TGF-β(1) and bFGF expression through the activation of AP-1 and NF-κB transcription factors in bovine mammary epithelial cells.
- Authors: Wu J, Ding Y, Wang J, Wang F
- Issue date: 2018 Apr