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dc.contributor.authorDötsch, Andreas
dc.contributor.authorSchniederjans, Monika
dc.contributor.authorKhaledi, Ariane
dc.contributor.authorHornischer, Klaus
dc.contributor.authorSchulz, Sebastian
dc.contributor.authorBielecka, Agata
dc.contributor.authorEckweiler, Denitsa
dc.contributor.authorPohl, Sarah
dc.contributor.authorHäussler, Susanne
dc.date.accessioned2015-11-17T14:55:00Zen
dc.date.available2015-11-17T14:55:00Zen
dc.date.issued2015en
dc.identifier.citationThe Pseudomonas aeruginosa Transcriptional Landscape Is Shaped by Environmental Heterogeneity and Genetic Variation. 2015, 6 (4):e00749 MBioen
dc.identifier.issn2150-7511en
dc.identifier.pmid26126853en
dc.identifier.doi10.1128/mBio.00749-15en
dc.identifier.urihttp://hdl.handle.net/10033/582294en
dc.description.abstractPhenotypic variability among bacteria depends on gene expression in response to different environments, and it also reflects differences in genomic structure. In this study, we analyzed transcriptome sequencing (RNA-seq) profiles of 151 Pseudomonas aeruginosa clinical isolates under standard laboratory conditions and of one P. aeruginosa type strain under 14 different environmental conditions. Our approach allowed dissection of the impact of the genetic background versus environmental cues on P. aeruginosa gene expression profiles and revealed that phenotypic variation was larger in response to changing environments than between genomically different isolates. We demonstrate that mutations within the global regulator LasR affect more than one trait (pleiotropy) and that the interaction between mutations (epistasis) shapes the P. aeruginosa phenotypic plasticity landscape. Because of pleiotropic and epistatic effects, average genotype and phenotype measures appeared to be uncorrelated in P. aeruginosa.
dc.language.isoenen
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/260276en
dc.rightsopenAccessen
dc.titleThe Pseudomonas aeruginosa Transcriptional Landscape Is Shaped by Environmental Heterogeneity and Genetic Variation.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, D-38124 Braunschweig, Germany.en
dc.identifier.journalmBioen
refterms.dateFOA2018-06-13T07:19:49Z
html.description.abstractPhenotypic variability among bacteria depends on gene expression in response to different environments, and it also reflects differences in genomic structure. In this study, we analyzed transcriptome sequencing (RNA-seq) profiles of 151 Pseudomonas aeruginosa clinical isolates under standard laboratory conditions and of one P. aeruginosa type strain under 14 different environmental conditions. Our approach allowed dissection of the impact of the genetic background versus environmental cues on P. aeruginosa gene expression profiles and revealed that phenotypic variation was larger in response to changing environments than between genomically different isolates. We demonstrate that mutations within the global regulator LasR affect more than one trait (pleiotropy) and that the interaction between mutations (epistasis) shapes the P. aeruginosa phenotypic plasticity landscape. Because of pleiotropic and epistatic effects, average genotype and phenotype measures appeared to be uncorrelated in P. aeruginosa.


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