Murine cytomegalovirus infection via the intranasal route offers a robust model of immunity upon mucosal CMV infection.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
AuthorsOduro, Jennifer D
Lemmermann, Niels A W
Marandu, Thomas F
Holzki, Julia K
MetadataShow full item record
AbstractCytomegalovirus (CMV) is a ubiquitous virus, causing the most common congenital infection in humans, yet a vaccine against this virus is not available. The experimental study of immunity against CMV in animal models of infection, such as the infection of mice with the mouse CMV (MCMV), has relied on systemic intraperitoneal infection protocols, although the infection naturally transmits by mucosal routes via body fluids containing CMV. To characterize the biology of infections by mucosal routes, we have compared the kinetics of virus replication, the latent viral load, and CD8 T cell responses in lymphoid organs upon experimental intranasal and intragastric infection to intraperitoneal infection of two unrelated mouse strains. We have observed that intranasal infection induces robust and persistent virus replication in lungs and salivary glands, but a poor one in the spleen. CD8 T cell responses were somewhat weaker than upon intraperitoneal infection, but showed similar kinetic profiles and phenotypes of antigen-specific cells. On the other hand, intragastric infection resulted in abortive or poor virus replication in all tested organs, and poor T cell responses to the virus, especially at late times after infection. Consistent with the T cell kinetics, the MCMV latent load was high in the lungs, but low in the spleen of intranasally infected mice and lowest in all tested organs upon intragastric infection. In conclusion, we show here that intranasal, but not intragastric infection of mice with MCMV represents a robust model to study short and long-term biology of CMV infection by a mucosal route.
CitationMurine cytomegalovirus infection via the intranasal route offers a robust model of immunity upon mucosal CMV infection. 2015: J. Gen. Virol.
AffiliationHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
JournalThe Journal of general virology
The following license files are associated with this item:
- Mucosal and parenteral vaccination against acute and latent murine cytomegalovirus (MCMV) infection by using an attenuated MCMV mutant.
- Authors: MacDonald MR, Li XY, Stenberg RM, Campbell AE, Virgin HW 4th
- Issue date: 1998 Jan
- Mucosal CD8+ T cell responses induced by an MCMV based vaccine vector confer protection against influenza challenge.
- Authors: Zheng X, Oduro JD, Boehme JD, Borkner L, Ebensen T, Heise U, Gereke M, Pils MC, Krmpotic A, Guzmán CA, Bruder D, Čičin-Šain L
- Issue date: 2019 Sep
- Systemic priming-boosting immunization with a trivalent plasmid DNA and inactivated murine cytomegalovirus (MCMV) vaccine provides long-term protection against viral replication following systemic or mucosal MCMV challenge.
- Authors: Morello CS, Ye M, Hung S, Kelley LA, Spector DH
- Issue date: 2005 Jan
- Persistent viral replication and the development of T-cell responses after intranasal infection by MCMV.
- Authors: Zhang S, Caldeira-Dantas S, Smith CJ, Snyder CM
- Issue date: 2019 Aug
- Murine Cytomegalovirus Spreads by Dendritic Cell Recirculation.
- Authors: Farrell HE, Bruce K, Lawler C, Oliveira M, Cardin R, Davis-Poynter N, Stevenson PG
- Issue date: 2017 Oct 3