Generation of anti-TLR2 intrabody mediating inhibition of macrophage surface TLR2 expression and TLR2-driven cell activation.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
AuthorsKirschning, Carsten J
MetadataShow full item record
AbstractToll-like receptor (TLR) 2 is a component of the innate immune system and senses specific pathogen associated molecular patterns (PAMPs) of both microbial and viral origin. Cell activation via TLR2 and other pattern recognition receptors (PRRs) contributes to sepsis pathology and chronic inflammation both relying on overamplification of an immune response. Intracellular antibodies expressed and retained inside the endoplasmatic reticulum (ER-intrabodies) are applied to block translocation of secreted and cell surface molecules from the ER to the cell surface resulting in functional inhibition of the target protein. Here we describe generation and application of a functional anti-TLR2 ER intrabody (alphaT2ib) which was generated from an antagonistic monoclonal antibody (mAb) towards human and murine TLR2 (T2.5) to inhibit the function of TLR2. alphaT2ib is a scFv fragment comprising the variable domain of the heavy chain and the variable domain of the light chain of mAb T2.5 linked together by a synthetic (Gly4Ser)3 amino acid sequence.
CitationGeneration of anti-TLR2 intrabody mediating inhibition of macrophage surface TLR2 expression and TLR2-driven cell activation. 2010, 10:31 BMC Biotechnol.
AffiliationHelmholtz Centre for infection research, Inhoffenstr. 7, D-38124 Braunschweig, Germany.
The following license files are associated with this item:
- Molecular cloning and characterization of a novel anti-TLR9 intrabody.
- Authors: Reimer E, Somplatzki S, Zegenhagen D, Hänel S, Fels A, Bollhorst T, Hovest LG, Bauer S, Kirschning CJ, Böldicke T
- Issue date: 2013 Sep
- Anti-WASP intrabodies inhibit inflammatory responses induced by Toll-like receptors 3, 7, and 9, in macrophages.
- Authors: Sakuma C, Sato M, Oshima T, Takenouchi T, Chiba J, Kitani H
- Issue date: 2015 Feb 27
- Specific binding of the WASP N-terminal domain to Btk is critical for TLR2 signaling in macrophages.
- Authors: Sakuma C, Sato M, Takenouchi T, Kitani H
- Issue date: 2015 Feb
- Activation of Toll-like receptors by Burkholderia pseudomallei.
- Authors: West TE, Ernst RK, Jansson-Hutson MJ, Skerrett SJ
- Issue date: 2008 Aug 8
- Antagonistic antibody prevents toll-like receptor 2-driven lethal shock-like syndromes.
- Authors: Meng G, Rutz M, Schiemann M, Metzger J, Grabiec A, Schwandner R, Luppa PB, Ebel F, Busch DH, Bauer S, Wagner H, Kirschning CJ
- Issue date: 2004 May