Surface-modified yeast cells: A novel eukaryotic carrier for oral application.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
AuthorsKenngott, Elisabeth E
Schmitt, Manfred J
MetadataShow full item record
AbstractThe effective targeting and subsequent binding of particulate carriers to M cells in Peyer's patches of the gut is a prerequisite for the development of oral delivery systems. We have established a novel carrier system based on cell surface expression of the β1-integrin binding domain of invasins derived from Yersinia enterocolitica and Yersinia pseudotuberculosis on the yeast Saccharomyces cerevisiae. All invasin derivatives were shown to be effectively expressed on the cell surface and recombinant yeast cells showed improved binding to both human HEp-2 cells and M-like cells in vitro. Among the different derivatives tested, the integrin-binding domain of Y. enterocolitica invasin proved to be the most effective and was able to target Peyer's patches in vivo. In conclusion, cell surface-modified yeasts might provide a novel bioadhesive, eukaryotic carrier system for efficient and targeted delivery of either antigens or drugs via the oral route.
CitationSurface-modified yeast cells: A novel eukaryotic carrier for oral application. 2016, 224:1-7 J Control Release
AffiliationHelmholtz Institute for Pharmaceutical Research Saarland (HIPS);Saarland University, Building A4.1, 66123 Saarbruecken, Germany.
The following license files are associated with this item:
- Toll-like receptor 2 is critical for induction of Reg3 beta expression and intestinal clearance of Yersinia pseudotuberculosis.
- Authors: Dessein R, Gironella M, Vignal C, Peyrin-Biroulet L, Sokol H, Secher T, Lacas-Gervais S, Gratadoux JJ, Lafont F, Dagorn JC, Ryffel B, Akira S, Langella P, Nùñez G, Sirard JC, Iovanna J, Simonet M, Chamaillard M
- Issue date: 2009 Jun
- Expression and distribution of beta1 integrins in in vitro-induced M cells: implications for Yersinia adhesion to Peyer's patch epithelium.
- Authors: Hamzaoui N, Kernéis S, Caliot E, Pringault E
- Issue date: 2004 Sep
- M-cell surface beta1 integrin expression and invasin-mediated targeting of Yersinia pseudotuberculosis to mouse Peyer's patch M cells.
- Authors: Clark MA, Hirst BH, Jepson MA
- Issue date: 1998 Mar
- Interaction of Yersinia enterocolitica with epithelial cells: invasin beyond invasion.
- Authors: Grassl GA, Bohn E, Müller Y, Bühler OT, Autenrieth IB
- Issue date: 2003 Apr
- Stability of liposomes in vitro and their uptake by rat Peyer's patches following oral administration.
- Authors: Aramaki Y, Tomizawa H, Hara T, Yachi K, Kikuchi H, Tsuchiya S
- Issue date: 1993 Aug