Surface-modified yeast cells: A novel eukaryotic carrier for oral application.

Kenngott, Elisabeth E; Kiefer, Ruth; Schneider-Daum, Nicole; Hamann, Alf; Schneider, Marc; Schmitt, Manfred J; Breinig, Frank

dc.contributor.author	Kenngott, Elisabeth E
dc.contributor.author	Kiefer, Ruth
dc.contributor.author	Schneider-Daum, Nicole
dc.contributor.author	Hamann, Alf
dc.contributor.author	Schneider, Marc
dc.contributor.author	Schmitt, Manfred J
dc.contributor.author	Breinig, Frank
dc.date.accessioned	2016-02-23T14:20:57Z	en
dc.date.available	2016-02-23T14:20:57Z	en
dc.date.issued	2016-02-28	en
dc.identifier.citation	Surface-modified yeast cells: A novel eukaryotic carrier for oral application. 2016, 224:1-7 J Control Release	en
dc.identifier.issn	1873-4995	en
dc.identifier.pmid	26763373	en
dc.identifier.doi	10.1016/j.jconrel.2015.12.054	en
dc.identifier.uri	http://hdl.handle.net/10033/596967	en
dc.description.abstract	The effective targeting and subsequent binding of particulate carriers to M cells in Peyer's patches of the gut is a prerequisite for the development of oral delivery systems. We have established a novel carrier system based on cell surface expression of the β1-integrin binding domain of invasins derived from Yersinia enterocolitica and Yersinia pseudotuberculosis on the yeast Saccharomyces cerevisiae. All invasin derivatives were shown to be effectively expressed on the cell surface and recombinant yeast cells showed improved binding to both human HEp-2 cells and M-like cells in vitro. Among the different derivatives tested, the integrin-binding domain of Y. enterocolitica invasin proved to be the most effective and was able to target Peyer's patches in vivo. In conclusion, cell surface-modified yeasts might provide a novel bioadhesive, eukaryotic carrier system for efficient and targeted delivery of either antigens or drugs via the oral route.
dc.language.iso	en	en
dc.title	Surface-modified yeast cells: A novel eukaryotic carrier for oral application.	en
dc.type	Article	en
dc.contributor.department	Helmholtz Institute for Pharmaceutical Research Saarland (HIPS);Saarland University, Building A4.1, 66123 Saarbruecken, Germany.	en
dc.identifier.journal	Journal of controlled release : official journal of the Controlled Release Society	en
refterms.dateFOA	2017-03-01T00:00:00Z
html.description.abstract	The effective targeting and subsequent binding of particulate carriers to M cells in Peyer's patches of the gut is a prerequisite for the development of oral delivery systems. We have established a novel carrier system based on cell surface expression of the β1-integrin binding domain of invasins derived from Yersinia enterocolitica and Yersinia pseudotuberculosis on the yeast Saccharomyces cerevisiae. All invasin derivatives were shown to be effectively expressed on the cell surface and recombinant yeast cells showed improved binding to both human HEp-2 cells and M-like cells in vitro. Among the different derivatives tested, the integrin-binding domain of Y. enterocolitica invasin proved to be the most effective and was able to target Peyer's patches in vivo. In conclusion, cell surface-modified yeasts might provide a novel bioadhesive, eukaryotic carrier system for efficient and targeted delivery of either antigens or drugs via the oral route.