Interferon-γ-inducible Rab20 regulates endosomal morphology and EGFR degradation in macrophages.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Gutierrez, Maximiliano Gabriel
MetadataShow full item record
AbstractLittle is known about the molecular players that regulate changes in the endocytic pathway during immune activation. Here we investigate the role of Rab20 in the endocytic pathway during activation of macrophages. Rab20 is associated with endocytic structures, but the function of this Rab GTPase in the endocytic pathway remains poorly characterized. We find that in macrophages, Rab20 expression and endosomal association significantly increase after interferon-γ (IFN-γ) treatment. Moreover, IFN-γ and Rab20 expression induce a dramatic enlargement of endosomes. These enlarged endosomes are the result of homotypic fusion promoted by Rab20 expression. The expression of Rab20 or the dominant-negative mutant Rab20T19N does not affect transferrin or dextran 70 kDa uptake. However, knockdown of Rab20 accelerates epidermal growth factor (EGF) trafficking to LAMP-2-positive compartments and EGF receptor degradation. Thus this work defines a function for Rab20 in the endocytic pathway during immune activation of macrophages.
CitationInterferon-γ-inducible Rab20 regulates endosomal morphology and EGFR degradation in macrophages. 2015, 26 (17):3061-70 Mol. Biol. Cell
AffiliationHelmholtz Centre for infection research (HZI), 38124 Braunschweig, Germany.
JournalMolecular biology of the cell
The following license files are associated with this item:
- Identification of an immune-regulated phagosomal Rab cascade in macrophages.
- Authors: Pei G, Repnik U, Griffiths G, Gutierrez MG
- Issue date: 2014 May 1
- Expression of dominant negative rab5 in HeLa cells regulates endocytic trafficking distal from the plasma membrane.
- Authors: Dinneen JL, Ceresa BP
- Issue date: 2004 Apr 1
- A Rab20-Dependent Membrane Trafficking Pathway Controls M. tuberculosis Replication by Regulating Phagosome Spaciousness and Integrity.
- Authors: Schnettger L, Rodgers A, Repnik U, Lai RP, Pei G, Verdoes M, Wilkinson RJ, Young DB, Gutierrez MG
- Issue date: 2017 May 10
- γ-SNAP stimulates disassembly of endosomal SNARE complexes and regulates endocytic trafficking pathways.
- Authors: Inoue H, Matsuzaki Y, Tanaka A, Hosoi K, Ichimura K, Arasaki K, Wakana Y, Asano K, Tanaka M, Okuzaki D, Yamamoto A, Tani K, Tagaya M
- Issue date: 2015 Aug 1
- The small GTPase Rab22 interacts with EEA1 and controls endosomal membrane trafficking.
- Authors: Kauppi M, Simonsen A, Bremnes B, Vieira A, Callaghan J, Stenmark H, Olkkonen VM
- Issue date: 2002 Mar 1