Interferon-γ-inducible Rab20 regulates endosomal morphology and EGFR degradation in macrophages.
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Authors
Pei, GangSchnettger, Laura
Bronietzki, Marc
Repnik, Urska
Griffiths, Gareth
Gutierrez, Maximiliano Gabriel
Issue Date
2015-09-01
Metadata
Show full item recordAbstract
Little is known about the molecular players that regulate changes in the endocytic pathway during immune activation. Here we investigate the role of Rab20 in the endocytic pathway during activation of macrophages. Rab20 is associated with endocytic structures, but the function of this Rab GTPase in the endocytic pathway remains poorly characterized. We find that in macrophages, Rab20 expression and endosomal association significantly increase after interferon-γ (IFN-γ) treatment. Moreover, IFN-γ and Rab20 expression induce a dramatic enlargement of endosomes. These enlarged endosomes are the result of homotypic fusion promoted by Rab20 expression. The expression of Rab20 or the dominant-negative mutant Rab20T19N does not affect transferrin or dextran 70 kDa uptake. However, knockdown of Rab20 accelerates epidermal growth factor (EGF) trafficking to LAMP-2-positive compartments and EGF receptor degradation. Thus this work defines a function for Rab20 in the endocytic pathway during immune activation of macrophages.Citation
Interferon-γ-inducible Rab20 regulates endosomal morphology and EGFR degradation in macrophages. 2015, 26 (17):3061-70 Mol. Biol. CellAffiliation
Helmholtz Centre for infection research (HZI), 38124 Braunschweig, Germany.Journal
Molecular biology of the cellPubMed ID
26157167Type
ArticleLanguage
enISSN
1939-4586ae974a485f413a2113503eed53cd6c53
10.1091/mbc.E14-11-1547
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