Interferon-γ-inducible Rab20 regulates endosomal morphology and EGFR degradation in macrophages.

Pei, Gang; Schnettger, Laura; Bronietzki, Marc; Repnik, Urska; Griffiths, Gareth; Gutierrez, Maximiliano Gabriel

dc.contributor.author	Pei, Gang
dc.contributor.author	Schnettger, Laura
dc.contributor.author	Bronietzki, Marc
dc.contributor.author	Repnik, Urska
dc.contributor.author	Griffiths, Gareth
dc.contributor.author	Gutierrez, Maximiliano Gabriel
dc.date.accessioned	2016-03-16T11:54:07Z	en
dc.date.available	2016-03-16T11:54:07Z	en
dc.date.issued	2015-09-01	en
dc.identifier.citation	Interferon-γ-inducible Rab20 regulates endosomal morphology and EGFR degradation in macrophages. 2015, 26 (17):3061-70 Mol. Biol. Cell	en
dc.identifier.issn	1939-4586	en
dc.identifier.pmid	26157167	en
dc.identifier.doi	10.1091/mbc.E14-11-1547	en
dc.identifier.uri	http://hdl.handle.net/10033/601377	en
dc.description.abstract	Little is known about the molecular players that regulate changes in the endocytic pathway during immune activation. Here we investigate the role of Rab20 in the endocytic pathway during activation of macrophages. Rab20 is associated with endocytic structures, but the function of this Rab GTPase in the endocytic pathway remains poorly characterized. We find that in macrophages, Rab20 expression and endosomal association significantly increase after interferon-γ (IFN-γ) treatment. Moreover, IFN-γ and Rab20 expression induce a dramatic enlargement of endosomes. These enlarged endosomes are the result of homotypic fusion promoted by Rab20 expression. The expression of Rab20 or the dominant-negative mutant Rab20T19N does not affect transferrin or dextran 70 kDa uptake. However, knockdown of Rab20 accelerates epidermal growth factor (EGF) trafficking to LAMP-2-positive compartments and EGF receptor degradation. Thus this work defines a function for Rab20 in the endocytic pathway during immune activation of macrophages.
dc.language.iso	en	en
dc.title	Interferon-γ-inducible Rab20 regulates endosomal morphology and EGFR degradation in macrophages.	en
dc.type	Article	en
dc.contributor.department	Helmholtz Centre for infection research (HZI), 38124 Braunschweig, Germany.	en
dc.identifier.journal	Molecular biology of the cell	en
refterms.dateFOA	2018-06-13T05:41:27Z
html.description.abstract	Little is known about the molecular players that regulate changes in the endocytic pathway during immune activation. Here we investigate the role of Rab20 in the endocytic pathway during activation of macrophages. Rab20 is associated with endocytic structures, but the function of this Rab GTPase in the endocytic pathway remains poorly characterized. We find that in macrophages, Rab20 expression and endosomal association significantly increase after interferon-γ (IFN-γ) treatment. Moreover, IFN-γ and Rab20 expression induce a dramatic enlargement of endosomes. These enlarged endosomes are the result of homotypic fusion promoted by Rab20 expression. The expression of Rab20 or the dominant-negative mutant Rab20T19N does not affect transferrin or dextran 70 kDa uptake. However, knockdown of Rab20 accelerates epidermal growth factor (EGF) trafficking to LAMP-2-positive compartments and EGF receptor degradation. Thus this work defines a function for Rab20 in the endocytic pathway during immune activation of macrophages.