Human alveolar epithelial cells expressing tight junctions to model the air-blood barrier.
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Authors
Kuehn, AnnaKletting, Stephanie
de Souza Carvalho-Wodarz, Cristiane
Repnik, Urska
Griffiths, Gareth
Fischer, Ulrike
Meese, Eckart
Huwer, Hanno
Wirth, Dagmar
May, Tobias
Schneider-Daum, Nicole
Lehr, Claus Michael

Issue Date
2016-03-17
Metadata
Show full item recordAbstract
This paper describes a new human alveolar epithelial cell line (hAELVi - human Alveolar Epithelial Lentivirus immortalized) with type I-like characteristics and functional tight junctions, suitable to model the air-blood barrier of the peripheral lung. Primary human alveolar epithelial cells were immortalized by a novel regimen, grown as monolayers on permeable filter supports and characterized morphologically, biochemically and biophysically. hAELVi cells maintain the capacity to form tight intercellular junctions, with high trans-epithelial electrical resistance (> 1000 Ω*cm²). The cells could be kept in culture over several days, up to passage 75, under liquid-liquid as well as air-liquid conditions. Ultrastructural analysis and real time PCR revealed type I-like cell properties, such as the presence of caveolae, expression of caveolin-1, and absence of surfactant protein C. Accounting for the barrier properties, inter-digitations sealed with tight junctions and desmosomes were also observed. Low permeability of the hydrophilic marker sodium fluorescein confirmed the suitability of hAELVi cells for in vitro transport studies across the alveolar epithelium. These results suggest that hAELVi cells reflect the essential features of the air-blood barrier, as needed for an alternative to animal testing to study absorption and toxicity of inhaled drugs, chemicals and nanomaterials.Citation
Human alveolar epithelial cells expressing tight junctions to model the air-blood barrier. 2016: ALTEXAffiliation
Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Saarland University, Campus C2.3, D-66123 Saarbrücken, Germany.Journal
ALTEXPubMed ID
26985677Type
ArticleISSN
1868-596Xae974a485f413a2113503eed53cd6c53
10.14573/altex.1511131
Scopus Count
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