Mutations improving production and secretion of extracellular lipase by Burkholderia glumae PG1.
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Authors
Knapp, AndreasVoget, Sonja
Gao, Rong
Zaburannyi, Nestor
Krysciak, Dagmar
Breuer, Michael
Hauer, Bernhard
Streit, Wolfgang R
Müller, Rolf

Daniel, Rolf
Jaeger, Karl-Erich
Issue Date
2015-10-17
Metadata
Show full item recordAbstract
Burkholderia glumae is a Gram-negative phytopathogenic bacterium known as the causative agent of rice panicle blight. Strain B. glumae PG1 is used for the production of a biotechnologically relevant lipase, which is secreted into the culture supernatant via a type II secretion pathway. We have comparatively analyzed the genome sequences of B. glumae PG1 wild type and a lipase overproducing strain obtained by classical strain mutagenesis. Among a total number of 72 single nucleotide polymorphisms (SNPs) identified in the genome of the production strain, two were localized in front of the lipAB operon and were analyzed in detail. Both mutations contribute to a 100-fold overproduction of extracellular lipase in B. glumae PG1 by affecting transcription of the lipAB operon and efficiency of lipase secretion. We analyzed each of the two SNPs separately and observed a stronger influence of the promoter mutation than of the signal peptide modification but also a cumulative effect of both mutations. Furthermore, fusion of the mutated LipA signal peptide resulted in a 2-fold increase in secretion of the heterologous reporter alkaline phosphatase from Escherichia coli.Citation
Mutations improving production and secretion of extracellular lipase by Burkholderia glumae PG1. 2015: Appl. Microbiol. Biotechnol.Affiliation
Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS),Saarland 9 University, 66123 Saarbrücken, Germany.PubMed ID
26476653Type
ArticleISSN
1432-0614ae974a485f413a2113503eed53cd6c53
10.1007/s00253-015-7041-z
Scopus Count
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