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dc.contributor.authorThiam, Hawa-Racine
dc.contributor.authorVargas, Pablo
dc.contributor.authorCarpi, Nicolas
dc.contributor.authorCrespo, Carolina Lage
dc.contributor.authorRaab, Matthew
dc.contributor.authorTerriac, Emmanuel
dc.contributor.authorKing, Megan C
dc.contributor.authorJacobelli, Jordan
dc.contributor.authorAlberts, Arthur S
dc.contributor.authorStradal, Theresia
dc.contributor.authorLennon-Dumenil, Ana-Maria
dc.contributor.authorPiel, Matthieu
dc.date.accessioned2016-05-03T13:57:47Zen
dc.date.available2016-05-03T13:57:47Zen
dc.date.issued2016en
dc.identifier.citationPerinuclear Arp2/3-driven actin polymerization enables nuclear deformation to facilitate cell migration through complex environments. 2016, 7:10997 Nat Communen
dc.identifier.issn2041-1723en
dc.identifier.pmid26975831en
dc.identifier.doi10.1038/ncomms10997en
dc.identifier.urihttp://hdl.handle.net/10033/607739en
dc.description.abstractCell migration has two opposite faces: although necessary for physiological processes such as immune responses, it can also have detrimental effects by enabling metastatic cells to invade new organs. In vivo, migration occurs in complex environments and often requires a high cellular deformability, a property limited by the cell nucleus. Here we show that dendritic cells, the sentinels of the immune system, possess a mechanism to pass through micrometric constrictions. This mechanism is based on a rapid Arp2/3-dependent actin nucleation around the nucleus that disrupts the nuclear lamina, the main structure limiting nuclear deformability. The cells' requirement for Arp2/3 to pass through constrictions can be relieved when nuclear stiffness is decreased by suppressing lamin A/C expression. We propose a new role for Arp2/3 in three-dimensional cell migration, allowing fast-moving cells such as leukocytes to rapidly and efficiently migrate through narrow gaps, a process probably important for their function.
dc.language.isoenen
dc.titlePerinuclear Arp2/3-driven actin polymerization enables nuclear deformation to facilitate cell migration through complex environments.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalNature communicationsen
refterms.dateFOA2018-06-13T09:04:49Z
html.description.abstractCell migration has two opposite faces: although necessary for physiological processes such as immune responses, it can also have detrimental effects by enabling metastatic cells to invade new organs. In vivo, migration occurs in complex environments and often requires a high cellular deformability, a property limited by the cell nucleus. Here we show that dendritic cells, the sentinels of the immune system, possess a mechanism to pass through micrometric constrictions. This mechanism is based on a rapid Arp2/3-dependent actin nucleation around the nucleus that disrupts the nuclear lamina, the main structure limiting nuclear deformability. The cells' requirement for Arp2/3 to pass through constrictions can be relieved when nuclear stiffness is decreased by suppressing lamin A/C expression. We propose a new role for Arp2/3 in three-dimensional cell migration, allowing fast-moving cells such as leukocytes to rapidly and efficiently migrate through narrow gaps, a process probably important for their function.


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