Integrated analysis of gene expression and metabolic fluxes in PHA-producing Pseudomonas putida grown on glycerol.

Beckers, Veronique; Poblete-Castro, Ignacio; Tomasch, Jürgen; Wittmann, Christoph

dc.contributor.author	Beckers, Veronique
dc.contributor.author	Poblete-Castro, Ignacio
dc.contributor.author	Tomasch, Jürgen
dc.contributor.author	Wittmann, Christoph
dc.date.accessioned	2016-06-07T13:47:24Z	en
dc.date.available	2016-06-07T13:47:24Z	en
dc.date.issued	2016	en
dc.identifier.citation	Integrated analysis of gene expression and metabolic fluxes in PHA-producing Pseudomonas putida grown on glycerol. 2016, 15 (1):73 Microb. Cell Fact.	en
dc.identifier.issn	1475-2859	en
dc.identifier.pmid	27142075	en
dc.identifier.doi	10.1186/s12934-016-0470-2	en
dc.identifier.uri	http://hdl.handle.net/10033/612003	en
dc.description.abstract	Given its high surplus and low cost, glycerol has emerged as interesting carbon substrate for the synthesis of value-added chemicals. The soil bacterium Pseudomonas putida KT2440 can use glycerol to synthesize medium-chain-length poly(3-hydroxyalkanoates) (mcl-PHA), a class of biopolymers of industrial interest. Here, glycerol metabolism in P. putida KT2440 was studied on the level of gene expression (transcriptome) and metabolic fluxes (fluxome), using precisely adjusted chemostat cultures, growth kinetics and stoichiometry, to gain a systematic understanding of the underlying metabolic and regulatory network.
dc.language.iso	en	en
dc.title	Integrated analysis of gene expression and metabolic fluxes in PHA-producing Pseudomonas putida grown on glycerol.	en
dc.type	Article	en
dc.contributor.department	Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.	en
dc.identifier.journal	Microbial cell factories	en
refterms.dateFOA	2018-06-13T01:02:24Z
html.description.abstract	Given its high surplus and low cost, glycerol has emerged as interesting carbon substrate for the synthesis of value-added chemicals. The soil bacterium Pseudomonas putida KT2440 can use glycerol to synthesize medium-chain-length poly(3-hydroxyalkanoates) (mcl-PHA), a class of biopolymers of industrial interest. Here, glycerol metabolism in P. putida KT2440 was studied on the level of gene expression (transcriptome) and metabolic fluxes (fluxome), using precisely adjusted chemostat cultures, growth kinetics and stoichiometry, to gain a systematic understanding of the underlying metabolic and regulatory network.