Alterations of miRNAs and miRNA-regulated mRNA expression in GC B cell lymphomas determined by integrative sequencing analysis.
dc.contributor.author | Hezaveh, Kebria | |
dc.contributor.author | Kloetgen, Andreas | |
dc.contributor.author | Bernhart, Stephan H | |
dc.contributor.author | Mahapatra, Kunal Das | |
dc.contributor.author | Lenze, Dido | |
dc.contributor.author | Richter, Julia | |
dc.contributor.author | Haake, Andrea | |
dc.contributor.author | Bergmann, Anke K | |
dc.contributor.author | Brors, Benedikt | |
dc.contributor.author | Burkhardt, Birgit | |
dc.contributor.author | Claviez, Alexander | |
dc.contributor.author | Drexler, Hans G | |
dc.contributor.author | Eils, Roland | |
dc.contributor.author | Haas, Siegfried | |
dc.contributor.author | Hoffmann, Steve | |
dc.contributor.author | Karsch, Dennis | |
dc.contributor.author | Klapper, Wolfram | |
dc.contributor.author | Kleinheinz, Kortine | |
dc.contributor.author | Korbel, Jan | |
dc.contributor.author | Kretzmer, Helene | |
dc.contributor.author | Kreuz, Markus | |
dc.contributor.author | Küppers, Ralf | |
dc.contributor.author | Lawerenz, Chris | |
dc.contributor.author | Leich, Ellen | |
dc.contributor.author | Loeffler, Markus | |
dc.contributor.author | Mantovani-Loeffler, Luisa | |
dc.contributor.author | López, Cristina | |
dc.contributor.author | McHardy, Alice C | |
dc.contributor.author | Möller, Peter | |
dc.contributor.author | Rohde, Marius | |
dc.contributor.author | Rosenstiel, Philip | |
dc.contributor.author | Rosenwald, Andreas | |
dc.contributor.author | Schilhabel, Markus | |
dc.contributor.author | Schlesner, Matthias | |
dc.contributor.author | Scholz, Ingrid | |
dc.contributor.author | Stadler, Peter F | |
dc.contributor.author | Stilgenbauer, Stephan | |
dc.contributor.author | Sungalee, Séphanie | |
dc.contributor.author | Szczepanowski, Monika | |
dc.contributor.author | Trümper, Lorenz | |
dc.contributor.author | Weniger, Marc A | |
dc.contributor.author | Siebert, Reiner | |
dc.contributor.author | Borkhardt, Arndt | |
dc.contributor.author | Hummel, Michael | |
dc.contributor.author | Hoell, Jessica I | |
dc.date.accessioned | 2016-07-21T10:51:35Z | |
dc.date.available | 2016-07-21T10:51:35Z | |
dc.date.issued | 2016-07-06 | |
dc.identifier.citation | Alterations of miRNAs and miRNA-regulated mRNA expression in GC B cell lymphomas determined by integrative sequencing analysis. 2016: Haematologica | en |
dc.identifier.issn | 1592-8721 | |
dc.identifier.pmid | 27390358 | |
dc.identifier.doi | 10.3324/haematol.2016.143891 | |
dc.identifier.uri | http://hdl.handle.net/10033/617314 | |
dc.description.abstract | MicroRNAs are well-established players in posttranscriptional gene regulation. However, information on the effects of microRNA deregulation mainly relies on bioinformatic prediction of potential targets, whereas proof of the direct physical microRNAs/target mRNAs interaction is mostly lacking. Within the International Cancer Genome Consortium Project Determining Molecular Mechanisms in Malignant Lymphoma by Sequencing (ICGC MMML-Seq), we performed miRnome sequencing from 16 Burkitt lymphomas, 19 diffuse large B-cell lymphomas, and 21 follicular lymphomas. Twenty-two miRNAs separated Burkitt lymphomas from diffuse large B-cell lymphomas/follicular lymphomas, of which 13 have shown regulation by MYC. Moreover, we show expression of three hitherto unreported microRNAs. Additionally, we detect recurrent mutations of hsa-miR-142 in diffuse large B-cell lymphomas and follicular lymphomas, and editing of the hsa-miR-376 cluster, providing evidence for microRNA editing in lymphomagenesis. To interrogate the direct physical interactions of microRNAs with mRNAs, we performed Argonaute-2 photoactivatable ribonucleoside-enhanced cross-linking and immunoprecipitation experiments. MicroRNAs directly targeted 208 mRNAs in the Burkitt lymphomas and 328 mRNAs in the non-Burkitt lymphoma models. This integrative analysis discovered several regulatory pathways of relevance in lymphomagenesis including Ras, PI3K-Akt and MAPK signaling pathways, also recurrently deregulated in lymphomas by mutations. Our dataset uncovers in detail the mRNA deregulation through microRNAs as a highly relevant mechanism in lymphomagenesis. | |
dc.language | ENG | |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | * |
dc.title | Alterations of miRNAs and miRNA-regulated mRNA expression in GC B cell lymphomas determined by integrative sequencing analysis. | |
dc.type | Article | en |
dc.contributor.department | Heinrich-Heine-University Duesseldorf, Medical Faculty, Department of Pediatric Oncology | en |
dc.identifier.journal | Haematologica | en |
refterms.dateFOA | 2018-06-12T23:13:59Z | |
html.description.abstract | MicroRNAs are well-established players in posttranscriptional gene regulation. However, information on the effects of microRNA deregulation mainly relies on bioinformatic prediction of potential targets, whereas proof of the direct physical microRNAs/target mRNAs interaction is mostly lacking. Within the International Cancer Genome Consortium Project Determining Molecular Mechanisms in Malignant Lymphoma by Sequencing (ICGC MMML-Seq), we performed miRnome sequencing from 16 Burkitt lymphomas, 19 diffuse large B-cell lymphomas, and 21 follicular lymphomas. Twenty-two miRNAs separated Burkitt lymphomas from diffuse large B-cell lymphomas/follicular lymphomas, of which 13 have shown regulation by MYC. Moreover, we show expression of three hitherto unreported microRNAs. Additionally, we detect recurrent mutations of hsa-miR-142 in diffuse large B-cell lymphomas and follicular lymphomas, and editing of the hsa-miR-376 cluster, providing evidence for microRNA editing in lymphomagenesis. To interrogate the direct physical interactions of microRNAs with mRNAs, we performed Argonaute-2 photoactivatable ribonucleoside-enhanced cross-linking and immunoprecipitation experiments. MicroRNAs directly targeted 208 mRNAs in the Burkitt lymphomas and 328 mRNAs in the non-Burkitt lymphoma models. This integrative analysis discovered several regulatory pathways of relevance in lymphomagenesis including Ras, PI3K-Akt and MAPK signaling pathways, also recurrently deregulated in lymphomas by mutations. Our dataset uncovers in detail the mRNA deregulation through microRNAs as a highly relevant mechanism in lymphomagenesis. |