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dc.contributor.authorHezaveh, Kebria
dc.contributor.authorKloetgen, Andreas
dc.contributor.authorBernhart, Stephan H
dc.contributor.authorMahapatra, Kunal Das
dc.contributor.authorLenze, Dido
dc.contributor.authorRichter, Julia
dc.contributor.authorHaake, Andrea
dc.contributor.authorBergmann, Anke K
dc.contributor.authorBrors, Benedikt
dc.contributor.authorBurkhardt, Birgit
dc.contributor.authorClaviez, Alexander
dc.contributor.authorDrexler, Hans G
dc.contributor.authorEils, Roland
dc.contributor.authorHaas, Siegfried
dc.contributor.authorHoffmann, Steve
dc.contributor.authorKarsch, Dennis
dc.contributor.authorKlapper, Wolfram
dc.contributor.authorKleinheinz, Kortine
dc.contributor.authorKorbel, Jan
dc.contributor.authorKretzmer, Helene
dc.contributor.authorKreuz, Markus
dc.contributor.authorKüppers, Ralf
dc.contributor.authorLawerenz, Chris
dc.contributor.authorLeich, Ellen
dc.contributor.authorLoeffler, Markus
dc.contributor.authorMantovani-Loeffler, Luisa
dc.contributor.authorLópez, Cristina
dc.contributor.authorMcHardy, Alice C
dc.contributor.authorMöller, Peter
dc.contributor.authorRohde, Marius
dc.contributor.authorRosenstiel, Philip
dc.contributor.authorRosenwald, Andreas
dc.contributor.authorSchilhabel, Markus
dc.contributor.authorSchlesner, Matthias
dc.contributor.authorScholz, Ingrid
dc.contributor.authorStadler, Peter F
dc.contributor.authorStilgenbauer, Stephan
dc.contributor.authorSungalee, Séphanie
dc.contributor.authorSzczepanowski, Monika
dc.contributor.authorTrümper, Lorenz
dc.contributor.authorWeniger, Marc A
dc.contributor.authorSiebert, Reiner
dc.contributor.authorBorkhardt, Arndt
dc.contributor.authorHummel, Michael
dc.contributor.authorHoell, Jessica I
dc.date.accessioned2016-07-21T10:51:35Z
dc.date.available2016-07-21T10:51:35Z
dc.date.issued2016-07-06
dc.identifier.citationAlterations of miRNAs and miRNA-regulated mRNA expression in GC B cell lymphomas determined by integrative sequencing analysis. 2016: Haematologicaen
dc.identifier.issn1592-8721
dc.identifier.pmid27390358
dc.identifier.doi10.3324/haematol.2016.143891
dc.identifier.urihttp://hdl.handle.net/10033/617314
dc.description.abstractMicroRNAs are well-established players in posttranscriptional gene regulation. However, information on the effects of microRNA deregulation mainly relies on bioinformatic prediction of potential targets, whereas proof of the direct physical microRNAs/target mRNAs interaction is mostly lacking. Within the International Cancer Genome Consortium Project Determining Molecular Mechanisms in Malignant Lymphoma by Sequencing (ICGC MMML-Seq), we performed miRnome sequencing from 16 Burkitt lymphomas, 19 diffuse large B-cell lymphomas, and 21 follicular lymphomas. Twenty-two miRNAs separated Burkitt lymphomas from diffuse large B-cell lymphomas/follicular lymphomas, of which 13 have shown regulation by MYC. Moreover, we show expression of three hitherto unreported microRNAs. Additionally, we detect recurrent mutations of hsa-miR-142 in diffuse large B-cell lymphomas and follicular lymphomas, and editing of the hsa-miR-376 cluster, providing evidence for microRNA editing in lymphomagenesis. To interrogate the direct physical interactions of microRNAs with mRNAs, we performed Argonaute-2 photoactivatable ribonucleoside-enhanced cross-linking and immunoprecipitation experiments. MicroRNAs directly targeted 208 mRNAs in the Burkitt lymphomas and 328 mRNAs in the non-Burkitt lymphoma models. This integrative analysis discovered several regulatory pathways of relevance in lymphomagenesis including Ras, PI3K-Akt and MAPK signaling pathways, also recurrently deregulated in lymphomas by mutations. Our dataset uncovers in detail the mRNA deregulation through microRNAs as a highly relevant mechanism in lymphomagenesis.
dc.languageENG
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleAlterations of miRNAs and miRNA-regulated mRNA expression in GC B cell lymphomas determined by integrative sequencing analysis.
dc.typeArticleen
dc.contributor.departmentHeinrich-Heine-University Duesseldorf, Medical Faculty, Department of Pediatric Oncologyen
dc.identifier.journalHaematologicaen
refterms.dateFOA2018-06-12T23:13:59Z
html.description.abstractMicroRNAs are well-established players in posttranscriptional gene regulation. However, information on the effects of microRNA deregulation mainly relies on bioinformatic prediction of potential targets, whereas proof of the direct physical microRNAs/target mRNAs interaction is mostly lacking. Within the International Cancer Genome Consortium Project Determining Molecular Mechanisms in Malignant Lymphoma by Sequencing (ICGC MMML-Seq), we performed miRnome sequencing from 16 Burkitt lymphomas, 19 diffuse large B-cell lymphomas, and 21 follicular lymphomas. Twenty-two miRNAs separated Burkitt lymphomas from diffuse large B-cell lymphomas/follicular lymphomas, of which 13 have shown regulation by MYC. Moreover, we show expression of three hitherto unreported microRNAs. Additionally, we detect recurrent mutations of hsa-miR-142 in diffuse large B-cell lymphomas and follicular lymphomas, and editing of the hsa-miR-376 cluster, providing evidence for microRNA editing in lymphomagenesis. To interrogate the direct physical interactions of microRNAs with mRNAs, we performed Argonaute-2 photoactivatable ribonucleoside-enhanced cross-linking and immunoprecipitation experiments. MicroRNAs directly targeted 208 mRNAs in the Burkitt lymphomas and 328 mRNAs in the non-Burkitt lymphoma models. This integrative analysis discovered several regulatory pathways of relevance in lymphomagenesis including Ras, PI3K-Akt and MAPK signaling pathways, also recurrently deregulated in lymphomas by mutations. Our dataset uncovers in detail the mRNA deregulation through microRNAs as a highly relevant mechanism in lymphomagenesis.


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