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dc.contributor.authorBruchmann, Sebastian
dc.contributor.authorMuthukumarasamy, Uthayakumar
dc.contributor.authorPohl, Sarah
dc.contributor.authorPreusse, Matthias
dc.contributor.authorBielecka, Agata
dc.contributor.authorNicolai, Tanja
dc.contributor.authorHamann, Isabell
dc.contributor.authorHillert, Roger
dc.contributor.authorKola, Axel
dc.contributor.authorGastmeier, Petra
dc.contributor.authorEckweiler, Denitsa
dc.contributor.authorHäussler, Susanne
dc.date.accessioned2016-08-11T10:59:08Z
dc.date.available2016-08-11T10:59:08Z
dc.date.issued2015-11
dc.identifier.citationDeep transcriptome profiling of clinical Klebsiella pneumoniae isolates reveals strain and sequence type-specific adaptation. 2015, 17 (11):4690-710 Environ. Microbiol.en
dc.identifier.issn1462-2920
dc.identifier.pmid26261087
dc.identifier.doi10.1111/1462-2920.13016
dc.identifier.urihttp://hdl.handle.net/10033/618242
dc.description.abstractHealth-care-associated infections by multi-drug-resistant bacteria constitute one of the greatest challenges to modern medicine. Bacterial pathogens devise various mechanisms to withstand the activity of a wide range of antimicrobial compounds, among which the acquisition of carbapenemases is one of the most concerning. In Klebsiella pneumoniae, the dissemination of the K. pneumoniae carbapenemase is tightly connected to the global spread of certain clonal lineages. Although antibiotic resistance is a key driver for the global distribution of epidemic high-risk clones, there seem to be other adaptive traits that may explain their success. Here, we exploited the power of deep transcriptome profiling (RNA-seq) to shed light on the transcriptomic landscape of 37 clinical K. pneumoniae isolates of diverse phylogenetic origins. We identified a large set of 3346 genes which was expressed in all isolates. While the core-transcriptome profiles varied substantially between groups of different sequence types, they were more homogenous among isolates of the same sequence type. We furthermore linked the detailed information on differentially expressed genes with the clinically relevant phenotypes of biofilm formation and bacterial virulence. This allowed for the identification of a diminished expression of biofilm-specific genes within the low biofilm producing ST258 isolates as a sequence type-specific trait.
dc.language.isoenen
dc.relationnfo:eu-repo/grantAgreement/EC/FP7/260276en
dc.rightsembargoedAccessen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subject.meshAnimalsen
dc.subject.meshAnti-Bacterial Agentsen
dc.subject.meshBacterial Proteinsen
dc.subject.meshBase Sequenceen
dc.subject.meshBiofilmsen
dc.subject.meshCross Infectionen
dc.subject.meshCross-Sectional Studiesen
dc.subject.meshDrug Resistance, Multiple, Bacterialen
dc.subject.meshGene Expression Profilingen
dc.subject.meshGenetic Variationen
dc.subject.meshHigh-Throughput Nucleotide Sequencingen
dc.subject.meshHumansen
dc.subject.meshKlebsiella pneumoniaeen
dc.subject.meshLarvaen
dc.subject.meshMolecular Sequence Dataen
dc.subject.meshMothsen
dc.subject.meshPhylogenyen
dc.subject.meshSequence Analysis, RNAen
dc.subject.meshbeta-Lactamasesen
dc.titleDeep transcriptome profiling of clinical Klebsiella pneumoniae isolates reveals strain and sequence type-specific adaptation.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalEnvironmental microbiologyen
refterms.dateFOA2016-11-01T00:00:00Z
html.description.abstractHealth-care-associated infections by multi-drug-resistant bacteria constitute one of the greatest challenges to modern medicine. Bacterial pathogens devise various mechanisms to withstand the activity of a wide range of antimicrobial compounds, among which the acquisition of carbapenemases is one of the most concerning. In Klebsiella pneumoniae, the dissemination of the K. pneumoniae carbapenemase is tightly connected to the global spread of certain clonal lineages. Although antibiotic resistance is a key driver for the global distribution of epidemic high-risk clones, there seem to be other adaptive traits that may explain their success. Here, we exploited the power of deep transcriptome profiling (RNA-seq) to shed light on the transcriptomic landscape of 37 clinical K. pneumoniae isolates of diverse phylogenetic origins. We identified a large set of 3346 genes which was expressed in all isolates. While the core-transcriptome profiles varied substantially between groups of different sequence types, they were more homogenous among isolates of the same sequence type. We furthermore linked the detailed information on differentially expressed genes with the clinically relevant phenotypes of biofilm formation and bacterial virulence. This allowed for the identification of a diminished expression of biofilm-specific genes within the low biofilm producing ST258 isolates as a sequence type-specific trait.


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