Screening and characterization of molecules that modulate the biological activity of IFNs-I.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Zapol'skii, Viktor A
Kaufmann, Dieter E
MetadataShow full item record
AbstractType I Interferons (IFNs-I) are species-specific glycoproteins which play an important role as primary defence against viral infections and that can also modulate the adaptive immune system. In some autoimmune diseases, interferons (IFNs) are over-produced. IFNs are widely used as biopharmaceuticals for a variety of cancer indications, chronic viral diseases, and for their immuno-modulatory action in patients with multiple sclerosis; therefore, increasing their therapeutic efficiency and decreasing their side effects is of high clinical value. In this sense, it is interesting to find molecules that can modulate the activity of IFNs. In order to achieve that, it was necessary to establish a simple, fast and robust assay to analyze numerous compounds simultaneously. We developed four reporter gene assays (RGAs) to identify IFN activity modulator compounds by using WISH-Mx2/EGFP, HeLa-Mx2/EGFP, A549-Mx2/EGFP, and HEp2-Mx2/EGFP reporter cell lines (RCLs). All of them present a Z' factor higher than 0.7. By using these RGAs, natural and synthetic compounds were analyzed simultaneously. A total of 442 compounds were studied by the Low Throughput Screening (LTS) assay using the four RCLs to discriminate between their inhibitory or enhancing effects on IFN activity. Some of them were characterized and 15 leads were identified. Finally, one promising candidate with enhancing effect on IFN-α/-β activity and five compounds with inhibitory effect were described.
CitationScreening and characterization of molecules that modulate the biological activity of IFNs-I. 2016, 233:6-16 J. Biotechnol.
AffiliationHelmholtz Centre for infection research, Inhoffenstr. 7,38124 Braunschweig, Germany.
JournalJournal of biotechnology
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-sa/4.0/
- WISH cell line: from the antiviral system to a novel reporter gene assay to test the potency of human IFN-α and IFN-β.
- Authors: Bürgi Mde L, Prieto C, Etcheverrigaray M, Kratje R, Oggero M, Bollati-Fogolín M
- Issue date: 2012 Jul 31
- Identification and characterization of human interferon alpha inhibitors through a WISH cell line-based reporter gene assay.
- Authors: Bürgi M, Hernández P, Cabrera M, Cerecetto H, González M, Kratje R, Raimondi A, Oggero M, Bollati-Fogolín M
- Issue date: 2020 Jan
- Validation of a HeLa Mx2/Luc reporter cell line for the quantification of human type I interferons.
- Authors: Seo YJ, Kim GH, Kwak HJ, Nam JS, Lee HJ, Suh SK, Baek KM, Sohn YW, Hong SH
- Issue date: 2009
- A novel reporter gene assay for interferons based on CHO-K1 cells.
- Authors: Smilović V, Caserman S, Fonda I, Gaberc-Porekar V, Menart V
- Issue date: 2008 Apr 20
- High throughput screening for small molecule enhancers of the interferon signaling pathway to drive next-generation antiviral drug discovery.
- Authors: Patel DA, Patel AC, Nolan WC, Zhang Y, Holtzman MJ
- Issue date: 2012