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dc.contributor.authorLee, Changhan
dc.contributor.authorWigren, Edvard
dc.contributor.authorTrček, Janja
dc.contributor.authorPeters, Verena
dc.contributor.authorKim, Jihong
dc.contributor.authorHasni, Muhammad Sharif
dc.contributor.authorNimtz, Manfred
dc.contributor.authorLindqvist, Ylva
dc.contributor.authorPark, Chankyu
dc.contributor.authorCurth, Ute
dc.contributor.authorLünsdorf, Heinrich
dc.contributor.authorRömling, Ute
dc.date.accessioned2016-09-05T11:50:09Z
dc.date.available2016-09-05T11:50:09Z
dc.date.issued2015-11
dc.identifier.citationA novel protein quality control mechanism contributes to heat shock resistance of worldwide-distributed Pseudomonas aeruginosa clone C strains. 2015, 17 (11):4511-26 Environ. Microbiol.en
dc.identifier.issn1462-2920
dc.identifier.pmid26014207
dc.identifier.doi10.1111/1462-2920.12915
dc.identifier.urihttp://hdl.handle.net/10033/619838
dc.description.abstractPseudomonas aeruginosa is a highly successful nosocomial pathogen capable of causing a wide variety of infections with clone C strains most prevalent worldwide. In this study, we initially characterize a molecular mechanism of survival unique to clone C strains. We identified a P. aeruginosa clone C-specific genomic island (PACGI-1) that contains the highly expressed small heat shock protein sHsp20c, the founding member of a novel subclass of class B bacterial small heat shock proteins. sHsp20c and adjacent gene products are involved in resistance against heat shock. Heat stable sHsp20c is unconventionally expressed in stationary phase in a wide temperature range from 20 to 42°C. Purified sHsp20c has characteristic features of small heat shock protein class B as it is monodisperse, forms sphere-like 24-meric oligomers and exhibits significant chaperone activity. As the P. aeruginosa clone C population is significantly more heat shock resistant than genetically unrelated P. aeruginosa strains without sHsp20c, the horizontally acquired shsp20c operon might contribute to the survival of worldwide-distributed clone C strains.
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subject.meshAmino Acid Sequenceen
dc.subject.meshBase Sequenceen
dc.subject.meshCross Infectionen
dc.subject.meshDNA, Bacterialen
dc.subject.meshGenomic Islandsen
dc.subject.meshHeat-Shock Proteinsen
dc.subject.meshHeat-Shock Responseen
dc.subject.meshHot Temperatureen
dc.subject.meshMolecular Sequence Dataen
dc.subject.meshPseudomonas aeruginosaen
dc.subject.meshSequence Analysis, DNAen
dc.titleA novel protein quality control mechanism contributes to heat shock resistance of worldwide-distributed Pseudomonas aeruginosa clone C strains.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7,38124 Braunschweig, Germany.en
dc.identifier.journalEnvironmental microbiologyen
refterms.dateFOA2018-06-12T22:14:55Z
html.description.abstractPseudomonas aeruginosa is a highly successful nosocomial pathogen capable of causing a wide variety of infections with clone C strains most prevalent worldwide. In this study, we initially characterize a molecular mechanism of survival unique to clone C strains. We identified a P. aeruginosa clone C-specific genomic island (PACGI-1) that contains the highly expressed small heat shock protein sHsp20c, the founding member of a novel subclass of class B bacterial small heat shock proteins. sHsp20c and adjacent gene products are involved in resistance against heat shock. Heat stable sHsp20c is unconventionally expressed in stationary phase in a wide temperature range from 20 to 42°C. Purified sHsp20c has characteristic features of small heat shock protein class B as it is monodisperse, forms sphere-like 24-meric oligomers and exhibits significant chaperone activity. As the P. aeruginosa clone C population is significantly more heat shock resistant than genetically unrelated P. aeruginosa strains without sHsp20c, the horizontally acquired shsp20c operon might contribute to the survival of worldwide-distributed clone C strains.


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