A novel protein quality control mechanism contributes to heat shock resistance of worldwide-distributed Pseudomonas aeruginosa clone C strains.
dc.contributor.author | Lee, Changhan | |
dc.contributor.author | Wigren, Edvard | |
dc.contributor.author | Trček, Janja | |
dc.contributor.author | Peters, Verena | |
dc.contributor.author | Kim, Jihong | |
dc.contributor.author | Hasni, Muhammad Sharif | |
dc.contributor.author | Nimtz, Manfred | |
dc.contributor.author | Lindqvist, Ylva | |
dc.contributor.author | Park, Chankyu | |
dc.contributor.author | Curth, Ute | |
dc.contributor.author | Lünsdorf, Heinrich | |
dc.contributor.author | Römling, Ute | |
dc.date.accessioned | 2016-09-05T11:50:09Z | |
dc.date.available | 2016-09-05T11:50:09Z | |
dc.date.issued | 2015-11 | |
dc.identifier.citation | A novel protein quality control mechanism contributes to heat shock resistance of worldwide-distributed Pseudomonas aeruginosa clone C strains. 2015, 17 (11):4511-26 Environ. Microbiol. | en |
dc.identifier.issn | 1462-2920 | |
dc.identifier.pmid | 26014207 | |
dc.identifier.doi | 10.1111/1462-2920.12915 | |
dc.identifier.uri | http://hdl.handle.net/10033/619838 | |
dc.description.abstract | Pseudomonas aeruginosa is a highly successful nosocomial pathogen capable of causing a wide variety of infections with clone C strains most prevalent worldwide. In this study, we initially characterize a molecular mechanism of survival unique to clone C strains. We identified a P. aeruginosa clone C-specific genomic island (PACGI-1) that contains the highly expressed small heat shock protein sHsp20c, the founding member of a novel subclass of class B bacterial small heat shock proteins. sHsp20c and adjacent gene products are involved in resistance against heat shock. Heat stable sHsp20c is unconventionally expressed in stationary phase in a wide temperature range from 20 to 42°C. Purified sHsp20c has characteristic features of small heat shock protein class B as it is monodisperse, forms sphere-like 24-meric oligomers and exhibits significant chaperone activity. As the P. aeruginosa clone C population is significantly more heat shock resistant than genetically unrelated P. aeruginosa strains without sHsp20c, the horizontally acquired shsp20c operon might contribute to the survival of worldwide-distributed clone C strains. | |
dc.language.iso | en | en |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | * |
dc.subject.mesh | Amino Acid Sequence | en |
dc.subject.mesh | Base Sequence | en |
dc.subject.mesh | Cross Infection | en |
dc.subject.mesh | DNA, Bacterial | en |
dc.subject.mesh | Genomic Islands | en |
dc.subject.mesh | Heat-Shock Proteins | en |
dc.subject.mesh | Heat-Shock Response | en |
dc.subject.mesh | Hot Temperature | en |
dc.subject.mesh | Molecular Sequence Data | en |
dc.subject.mesh | Pseudomonas aeruginosa | en |
dc.subject.mesh | Sequence Analysis, DNA | en |
dc.title | A novel protein quality control mechanism contributes to heat shock resistance of worldwide-distributed Pseudomonas aeruginosa clone C strains. | en |
dc.type | Article | en |
dc.contributor.department | Helmholtz Centre for infection research, Inhoffenstr. 7,38124 Braunschweig, Germany. | en |
dc.identifier.journal | Environmental microbiology | en |
refterms.dateFOA | 2018-06-12T22:14:55Z | |
html.description.abstract | Pseudomonas aeruginosa is a highly successful nosocomial pathogen capable of causing a wide variety of infections with clone C strains most prevalent worldwide. In this study, we initially characterize a molecular mechanism of survival unique to clone C strains. We identified a P. aeruginosa clone C-specific genomic island (PACGI-1) that contains the highly expressed small heat shock protein sHsp20c, the founding member of a novel subclass of class B bacterial small heat shock proteins. sHsp20c and adjacent gene products are involved in resistance against heat shock. Heat stable sHsp20c is unconventionally expressed in stationary phase in a wide temperature range from 20 to 42°C. Purified sHsp20c has characteristic features of small heat shock protein class B as it is monodisperse, forms sphere-like 24-meric oligomers and exhibits significant chaperone activity. As the P. aeruginosa clone C population is significantly more heat shock resistant than genetically unrelated P. aeruginosa strains without sHsp20c, the horizontally acquired shsp20c operon might contribute to the survival of worldwide-distributed clone C strains. |