Adaptation in CRISPR-Cas Systems.
dc.contributor.author | Sternberg, Samuel H | |
dc.contributor.author | Richter, Hagen | |
dc.contributor.author | Charpentier, Emmanuelle | |
dc.contributor.author | Qimron, Udi | |
dc.date.accessioned | 2016-09-14T08:13:12Z | |
dc.date.available | 2016-09-14T08:13:12Z | |
dc.date.issued | 2016-03-17 | |
dc.identifier.citation | Adaptation in CRISPR-Cas Systems. 2016, 61 (6):797-808 Mol. Cell | en |
dc.identifier.issn | 1097-4164 | |
dc.identifier.pmid | 26949040 | |
dc.identifier.doi | 10.1016/j.molcel.2016.01.030 | |
dc.identifier.uri | http://hdl.handle.net/10033/620134 | |
dc.description.abstract | Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins constitute an adaptive immune system in prokaryotes. The system preserves memories of prior infections by integrating short segments of foreign DNA, termed spacers, into the CRISPR array in a process termed adaptation. During the past 3 years, significant progress has been made on the genetic requirements and molecular mechanisms of adaptation. Here we review these recent advances, with a focus on the experimental approaches that have been developed, the insights they generated, and a proposed mechanism for self- versus non-self-discrimination during the process of spacer selection. We further describe the regulation of adaptation and the protein players involved in this fascinating process that allows bacteria and archaea to harbor adaptive immunity. | |
dc.language.iso | en | en |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | * |
dc.subject.mesh | Adaptive Immunity | en |
dc.subject.mesh | Archaea | en |
dc.subject.mesh | Bacteria | en |
dc.subject.mesh | CRISPR-Cas Systems | en |
dc.subject.mesh | Clustered Regularly Interspaced Short Palindromic Repeats | en |
dc.subject.mesh | Prokaryotic Cells | en |
dc.title | Adaptation in CRISPR-Cas Systems. | en |
dc.type | Article | en |
dc.contributor.department | Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany. | en |
dc.identifier.journal | Molecular cell | en |
refterms.dateFOA | 2017-03-15T00:00:00Z | |
html.description.abstract | Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins constitute an adaptive immune system in prokaryotes. The system preserves memories of prior infections by integrating short segments of foreign DNA, termed spacers, into the CRISPR array in a process termed adaptation. During the past 3 years, significant progress has been made on the genetic requirements and molecular mechanisms of adaptation. Here we review these recent advances, with a focus on the experimental approaches that have been developed, the insights they generated, and a proposed mechanism for self- versus non-self-discrimination during the process of spacer selection. We further describe the regulation of adaptation and the protein players involved in this fascinating process that allows bacteria and archaea to harbor adaptive immunity. |
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publications of the department Regulation of infection [12]
Leiter: Frau Prof. Dr. Emmanuelle Charpentier