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dc.contributor.authorSternberg, Samuel H
dc.contributor.authorRichter, Hagen
dc.contributor.authorCharpentier, Emmanuelle
dc.contributor.authorQimron, Udi
dc.date.accessioned2016-09-14T08:13:12Z
dc.date.available2016-09-14T08:13:12Z
dc.date.issued2016-03-17
dc.identifier.citationAdaptation in CRISPR-Cas Systems. 2016, 61 (6):797-808 Mol. Cellen
dc.identifier.issn1097-4164
dc.identifier.pmid26949040
dc.identifier.doi10.1016/j.molcel.2016.01.030
dc.identifier.urihttp://hdl.handle.net/10033/620134
dc.description.abstractClustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins constitute an adaptive immune system in prokaryotes. The system preserves memories of prior infections by integrating short segments of foreign DNA, termed spacers, into the CRISPR array in a process termed adaptation. During the past 3 years, significant progress has been made on the genetic requirements and molecular mechanisms of adaptation. Here we review these recent advances, with a focus on the experimental approaches that have been developed, the insights they generated, and a proposed mechanism for self- versus non-self-discrimination during the process of spacer selection. We further describe the regulation of adaptation and the protein players involved in this fascinating process that allows bacteria and archaea to harbor adaptive immunity.
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subject.meshAdaptive Immunityen
dc.subject.meshArchaeaen
dc.subject.meshBacteriaen
dc.subject.meshCRISPR-Cas Systemsen
dc.subject.meshClustered Regularly Interspaced Short Palindromic Repeatsen
dc.subject.meshProkaryotic Cellsen
dc.titleAdaptation in CRISPR-Cas Systems.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalMolecular cellen
refterms.dateFOA2017-03-15T00:00:00Z
html.description.abstractClustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins constitute an adaptive immune system in prokaryotes. The system preserves memories of prior infections by integrating short segments of foreign DNA, termed spacers, into the CRISPR array in a process termed adaptation. During the past 3 years, significant progress has been made on the genetic requirements and molecular mechanisms of adaptation. Here we review these recent advances, with a focus on the experimental approaches that have been developed, the insights they generated, and a proposed mechanism for self- versus non-self-discrimination during the process of spacer selection. We further describe the regulation of adaptation and the protein players involved in this fascinating process that allows bacteria and archaea to harbor adaptive immunity.


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