Adaptation in CRISPR-Cas Systems.

Sternberg, Samuel H; Richter, Hagen; Charpentier, Emmanuelle; Qimron, Udi

dc.contributor.author	Sternberg, Samuel H
dc.contributor.author	Richter, Hagen
dc.contributor.author	Charpentier, Emmanuelle
dc.contributor.author	Qimron, Udi
dc.date.accessioned	2016-09-14T08:13:12Z
dc.date.available	2016-09-14T08:13:12Z
dc.date.issued	2016-03-17
dc.identifier.citation	Adaptation in CRISPR-Cas Systems. 2016, 61 (6):797-808 Mol. Cell	en
dc.identifier.issn	1097-4164
dc.identifier.pmid	26949040
dc.identifier.doi	10.1016/j.molcel.2016.01.030
dc.identifier.uri	http://hdl.handle.net/10033/620134
dc.description.abstract	Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins constitute an adaptive immune system in prokaryotes. The system preserves memories of prior infections by integrating short segments of foreign DNA, termed spacers, into the CRISPR array in a process termed adaptation. During the past 3 years, significant progress has been made on the genetic requirements and molecular mechanisms of adaptation. Here we review these recent advances, with a focus on the experimental approaches that have been developed, the insights they generated, and a proposed mechanism for self- versus non-self-discrimination during the process of spacer selection. We further describe the regulation of adaptation and the protein players involved in this fascinating process that allows bacteria and archaea to harbor adaptive immunity.
dc.language.iso	en	en
dc.rights.uri	http://creativecommons.org/licenses/by-nc-sa/4.0/	*
dc.subject.mesh	Adaptive Immunity	en
dc.subject.mesh	Archaea	en
dc.subject.mesh	Bacteria	en
dc.subject.mesh	CRISPR-Cas Systems	en
dc.subject.mesh	Clustered Regularly Interspaced Short Palindromic Repeats	en
dc.subject.mesh	Prokaryotic Cells	en
dc.title	Adaptation in CRISPR-Cas Systems.	en
dc.type	Article	en
dc.contributor.department	Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.	en
dc.identifier.journal	Molecular cell	en
refterms.dateFOA	2017-03-15T00:00:00Z
html.description.abstract	Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins constitute an adaptive immune system in prokaryotes. The system preserves memories of prior infections by integrating short segments of foreign DNA, termed spacers, into the CRISPR array in a process termed adaptation. During the past 3 years, significant progress has been made on the genetic requirements and molecular mechanisms of adaptation. Here we review these recent advances, with a focus on the experimental approaches that have been developed, the insights they generated, and a proposed mechanism for self- versus non-self-discrimination during the process of spacer selection. We further describe the regulation of adaptation and the protein players involved in this fascinating process that allows bacteria and archaea to harbor adaptive immunity.