Subclones in B-lymphoma cell lines: isogenic models for the study of gene regulation.
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Authors
Quentmeier, HilmarPommerenke, Claudia

Ammerpohl, Ole
Geffers, Robert

Hauer, Vivien
MacLeod, Roderick Af
Nagel, Stefan
Romani, Julia
Rosati, Emanuela
Rosén, Anders
Uphoff, Cord C
Zaborski, Margarete
Drexler, Hans G
Issue Date
2016-08-23
Metadata
Show full item recordAbstract
Genetic heterogeneity though common in tumors has been rarely documented in cell lines. To examine how often B-lymphoma cell lines are comprised of subclones, we performed immunoglobulin (IG) heavy chain hypermutation analysis. Revealing that subclones are not rare in B-cell lymphoma cell lines, 6/49 IG hypermutated cell lines (12%) consisted of subclones with individual IG mutations. Subclones were also identified in 2/284 leukemia/lymphoma cell lines exhibiting bimodal CD marker expression. We successfully isolated 10 subclones from four cell lines (HG3, SU-DHL-5, TMD-8, U-2932). Whole exome sequencing was performed to molecularly characterize these subclones. We describe in detail the clonal structure of cell line HG3, derived from chronic lymphocytic leukemia. HG3 consists of three subclones each bearing clone-specific aberrations, gene expression and DNA methylation patterns. While donor patient leukemic cells were CD5+, two of three HG3 subclones had independently lost this marker. CD5 on HG3 cells was regulated by epigenetic/transcriptional mechanisms rather than by alternative splicing as reported hitherto. In conclusion, we show that the presence of subclones in cell lines carrying individual mutations and characterized by sets of differentially expressed genes is not uncommon. We show also that these subclones can be useful isogenic models for regulatory and functional studies.Citation
Subclones in B-lymphoma cell lines: isogenic models for the study of gene regulation. 2016: OncotargetAffiliation
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig.Journal
OncotargetPubMed ID
27566572Type
ArticleISSN
1949-2553ae974a485f413a2113503eed53cd6c53
10.18632/oncotarget.11524
Scopus Count
The following license files are associated with this item:
- Creative Commons
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-sa/4.0/
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