• Peptide-mediated interference with influenza A virus polymerase.

      Ghanem, Alexander; Mayer, Daniel; Chase, Geoffrey; Tegge, Werner; Frank, Ronald; Kochs, Georg; García-Sastre, Adolfo; Schwemmle, Martin; Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany. (2007-07)
      The assembly of the polymerase complex of influenza A virus from the three viral polymerase subunits PB1, PB2, and PA is required for viral RNA synthesis. We show that peptides which specifically bind to the protein-protein interaction domains in the subunits responsible for complex formation interfere with polymerase complex assembly and inhibit viral replication. Specifically, we provide evidence that a 25-amino-acid peptide corresponding to the PA-binding domain of PB1 blocks the polymerase activity of influenza A virus and inhibits viral spread. Targeting polymerase subunit interactions therefore provides a novel strategy to develop antiviral compounds against influenza A virus or other viruses.