Lymphatic endothelial cells are a replicative niche for Mycobacterium tuberculosis.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
AuthorsLerner, Thomas R
de Souza Carvalho-Wodarz, Cristiane
Russell, Matthew R G
Diedrich, Collin R
Collinson, Lucy M
Wilkinson, Robert J
Gutierrez, Maximiliano G
MetadataShow full item record
AbstractIn extrapulmonary tuberculosis, the most common site of infection is within the lymphatic system, and there is growing recognition that lymphatic endothelial cells (LECs) are involved in immune function. Here, we identified LECs, which line the lymphatic vessels, as a niche for Mycobacterium tuberculosis in the lymph nodes of patients with tuberculosis. In cultured primary human LECs (hLECs), we determined that M. tuberculosis replicates both in the cytosol and within autophagosomes, but the bacteria failed to replicate when the virulence locus RD1 was deleted. Activation by IFN-γ induced a cell-autonomous response in hLECs via autophagy and NO production that restricted M. tuberculosis growth. Thus, depending on the activation status of LECs, autophagy can both promote and restrict replication. Together, these findings reveal a previously unrecognized role for hLECs and autophagy in tuberculosis pathogenesis and suggest that hLECs are a potential niche for M. tuberculosis that allows establishment of persistent infection in lymph nodes.
CitationLymphatic endothelial cells are a replicative niche for Mycobacterium tuberculosis. 2016, 126 (3):1093-108 J. Clin. Invest.
AffiliationHelmholtz-Institute for Pharmaceutical Research Saarland (HIPS),Saarland Universitätscampus E8.1, 66123 Saarbrücken, Germany.
The following license files are associated with this item:
- Creative Commons
- Mycobacterium tuberculosis cords within lymphatic endothelial cells to evade host immunity.
- Authors: Lerner TR, Queval CJ, Lai RP, Russell MR, Fearns A, Greenwood DJ, Collinson L, Wilkinson RJ, Gutierrez MG
- Issue date: 2020 May 21
- Endothelial lineage-specific interaction of Mycobacterium tuberculosis with the blood and lymphatic systems.
- Authors: Kim PM, Lee JJ, Choi D, Eoh H, Hong YK
- Issue date: 2018 Jul
- Phthiocerol dimycocerosates promote access to the cytosol and intracellular burden of Mycobacterium tuberculosis in lymphatic endothelial cells.
- Authors: Lerner TR, Queval CJ, Fearns A, Repnik U, Griffiths G, Gutierrez MG
- Issue date: 2018 Jan 4
- Innate response of human endothelial cells infected with mycobacteria.
- Authors: García-Pérez BE, Villagómez-Palatto DA, Castañeda-Sánchez JI, Coral-Vázquez RM, Ramírez-Sánchez I, Ordoñez-Razo RM, Luna-Herrera J
- Issue date: 2011 Aug
- Reduced in vivo cytotoxicity and increased Mycobacterial burden are associated with virulent Mycobacterium tuberculosis strains during lung infection.
- Authors: Quintero-Macías L, Silva-Sánchez A, Valderrabano-Ortíz E, Munguía-Fuentes R, Aguilar-León D, Hernández-Pando R, Flores-Romo L
- Issue date: 2012