• Cellular delivery of polynucleotides by cationic cyclodextrin polyrotaxanes.

      Dandekar, Prajakta; Jain, Ratnesh; Keil, Manuel; Loretz, Brigitta; Muijs, Leon; Schneider, Marc; Auerbach, Dagmar; Jung, Gregor; Lehr, Claus-Michael; Wenz, Gerhard; et al. (2012-12-28)
      Cationic polyrotaxanes, obtained by temperature activated threading of cationic cyclodextrin derivatives onto water-soluble cationic polymers (ionenes), form metastable nanometric polyplexes with pDNA and combinations of siRNA with pDNA. Because of their low toxicity, the polyrotaxane polyplexes constitute a very interesting system for the transfection of polynucleotides into mammalian cells. The complexation of Cy3-labeled siRNA within the polyplexes was demonstrated by fluorescence correlation spectroscopy. The uptake of the polyplexes (red) was imaged by confocal fluorescence microscopy using the A549 cell line as a model (blue: nuclei, green: membranes). The results prove the potential of polyrotaxanes for further investigations involving knocking down genes of therapeutic interest.