• Identification of miRNAs associated with dendritic cell dysfunction during Acute and Chronic Hepatitis B virus infection.

      Singh, Avishek Kumar; Rooge, Sheetalnath Babasaheb; Varshney, Aditi; Vasudevan, Madavan; Kumar, Manoj; Geffers, Robert; Kumar, Vijay; Sarin, Shiv Kumar; HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany. (Wiley, 2020-10-27)
      The uptake or expression of hepatitis B virus proteins by Dendritic cells (DCs) is considered important for disease outcome. Differential expression of microRNA may have a role in viral persistence and hepatocellular injury. The miRNA expression was investigated by microarray in DCs from different stages of HBV infection and liver disease viz., immune active (IA; n=20); low replicative (LR; n=20); HBeAg negative (n=20); acute viral hepatitis (AVH, n=20) and healthy controls (n=20). miRNA levels were analyzed by unsupervised hierarchical clustering and principal component analyses and validated by qPCR. The miRNA-mRNA regulatory networks identified 19 miRNAs and 12 target gene interactions in MHC and other immune pathways. miR-2278, miR-615-3p and miR-3681-3p were down-regulated in IA group compared to healthy control, miR-152-3p and miR-3613-3p in LR group compared to IA group and miR-152-3p and miR-503-3p in HBe negative compared to LR group. However, miR-7-1-1-3p, miR-192-5p, miR-195-5p and miR-32-5p in LR, miR-342-3p and miR-940 in HBe negative, and miR-34a-5p, miR-130b-3p, miR-221-3p, miR-320a, miR-324-5p and miR-484 in AVH were up-regulated. Further, qPCR confirmed changes in miRNA levels and their target genes associated with antigen processing and presentation. Thus, a deregulated network of miRNAs-mRNAs in DCs seems responsible for impaired immune response during HBV pathogenesis. This article is protected by copyright. All rights reserved.