• PspA can form large scaffolds in Escherichia coli.

      Standar, Kerstin; Mehner, Denise; Osadnik, Hendrik; Berthelmann, Felix; Hause, Gerd; Lünsdorf, Heinrich; Brüser, Thomas; Institute of Biology/Microbiology, University of Halle-Wittenberg, Kurt-Mothes-Strasse 3, D-06120 Halle, Germany. (2008-10-29)
      The phage shock protein A (PspA) of Escherichia coli stabilizes the cytoplasmic membrane under stress conditions. Here we demonstrate that PspA can form hollow spherical or prolate spheroidal particles of about 30-40nm diameter with a scaffold-like arrangement of protein subunits at the surface. The 'PspA-scaffold' is the basic structure that is common to all particles. The PspA-scaffold may be of fundamental importance, as it could allow PspA to stabilize the integrity of membranes through numerous contact points over a large surface area.