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dc.contributor.authorKunze, Brigitte
dc.contributor.authorReck, Michael
dc.contributor.authorDötsch, Andreas
dc.contributor.authorLemme, André
dc.contributor.authorSchummer, Dietmar
dc.contributor.authorIrschik, Herbert
dc.contributor.authorSteinmetz, Heinrich
dc.contributor.authorWagner-Döbler, Irene
dc.date.accessioned2017-01-17T10:41:51Z
dc.date.available2017-01-17T10:41:51Z
dc.date.issued2010-07-26en
dc.identifier.citationBMC Microbiology. 2010 Jul 26;10(1):199en
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2180-10-199en
dc.identifier.urihttp://hdl.handle.net/10033/620720
dc.description.abstractAbstract Background Streptococcus mutans is a major pathogen in human dental caries. One of its important virulence properties is the ability to form biofilms (dental plaque) on tooth surfaces. Eradication of such biofilms is extremely difficult. We therefore screened a library of secondary metabolites from myxobacteria for their ability to damage biofilms of S. mutans. Results Here we show that carolacton, a secondary metabolite isolated from Sorangium cellulosum, has high antibacterial activity against biofilms of S. mutans. Planktonic growth of bacteria was only slightly impaired and no acute cytotoxicity against mouse fibroblasts could be observed. Carolacton caused death of S. mutans biofilm cells, elongation of cell chains, and changes in cell morphology. At a concentration of 10 nM carolacton, biofilm damage was already at 35% under anaerobic conditions. A knock-out mutant for comD, encoding a histidine kinase specific for the competence stimulating peptide (CSP), was slightly less sensitive to carolacton than the wildtype. Expression of the competence related alternate sigma factor ComX was strongly reduced by carolacton, as determined by a pcomX luciferase reporter strain. Conclusions Carolacton possibly interferes with the density dependent signalling systems in S. mutans and may represent a novel approach for the prevention of dental caries.
dc.titleDamage of Streptococcus mutans biofilms by carolacton, a secondary metabolite from the myxobacterium Sorangium cellulosumen
dc.typeJournal Articleen
dc.language.rfc3066enen
dc.rights.holderKunze et al.en
dc.date.updated2015-09-04T08:28:51Zen
refterms.dateFOA2018-06-13T02:30:55Z
html.description.abstractAbstract Background Streptococcus mutans is a major pathogen in human dental caries. One of its important virulence properties is the ability to form biofilms (dental plaque) on tooth surfaces. Eradication of such biofilms is extremely difficult. We therefore screened a library of secondary metabolites from myxobacteria for their ability to damage biofilms of S. mutans. Results Here we show that carolacton, a secondary metabolite isolated from Sorangium cellulosum, has high antibacterial activity against biofilms of S. mutans. Planktonic growth of bacteria was only slightly impaired and no acute cytotoxicity against mouse fibroblasts could be observed. Carolacton caused death of S. mutans biofilm cells, elongation of cell chains, and changes in cell morphology. At a concentration of 10 nM carolacton, biofilm damage was already at 35% under anaerobic conditions. A knock-out mutant for comD, encoding a histidine kinase specific for the competence stimulating peptide (CSP), was slightly less sensitive to carolacton than the wildtype. Expression of the competence related alternate sigma factor ComX was strongly reduced by carolacton, as determined by a pcomX luciferase reporter strain. Conclusions Carolacton possibly interferes with the density dependent signalling systems in S. mutans and may represent a novel approach for the prevention of dental caries.


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