Virus-like particle production with yeast: ultrastructural and immunocytochemical insights into Pichia pastoris producing high levels of the hepatitis B surface antigen.

Lünsdorf, Heinrich; Gurramkonda, Chandrasekhar; Adnan, Ahmad; Khanna, Navin; Rinas, Ursula

dc.contributor.author	Lünsdorf, Heinrich
dc.contributor.author	Gurramkonda, Chandrasekhar
dc.contributor.author	Adnan, Ahmad
dc.contributor.author	Khanna, Navin
dc.contributor.author	Rinas, Ursula
dc.date.accessioned	2017-01-24T14:40:32Z
dc.date.available	2017-01-24T14:40:32Z
dc.date.issued	2011-06-26
dc.identifier.citation	Virus-like particle production with yeast: ultrastructural and immunocytochemical insights into Pichia pastoris producing high levels of the hepatitis B surface antigen. 2011, 10:48 Microb. Cell Fact.	en
dc.identifier.issn	1475-2859
dc.identifier.pmid	21703024
dc.identifier.doi	10.1186/1475-2859-10-48
dc.identifier.uri	http://hdl.handle.net/10033/620742
dc.description.abstract	A protective immune response against Hepatitis B infection can be obtained through the administration of a single viral polypeptide, the Hepatitis B surface antigen (HBsAg). Thus, the Hepatitis B vaccine is generated through the utilization of recombinant DNA technology, preferentially by using yeast-based expression systems. However, the polypeptide needs to assemble into spherical particles, so-called virus-like particles (VLPs), to elicit the required protective immune response. So far, no clear evidence has been presented showing whether HBsAg assembles in vivo inside the yeast cell into VLPs or later in vitro during down-stream processing and purification.
dc.language.iso	en	en
dc.subject.mesh	Aldehyde Oxidase	en
dc.subject.mesh	Endoplasmic Reticulum	en
dc.subject.mesh	Hepatitis B Surface Antigens	en
dc.subject.mesh	Immunohistochemistry	en
dc.subject.mesh	Methanol	en
dc.subject.mesh	Microscopy, Electron, Transmission	en
dc.subject.mesh	Pichia	en
dc.subject.mesh	Vaccines, Synthetic	en
dc.subject.mesh	Vaccines, Virus-Like Particle	en
dc.title	Virus-like particle production with yeast: ultrastructural and immunocytochemical insights into Pichia pastoris producing high levels of the hepatitis B surface antigen.	en
dc.type	Article	en
dc.contributor.department	Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.	en
dc.identifier.journal	Microbial cell factories	en
refterms.dateFOA	2018-06-13T00:30:37Z
html.description.abstract	A protective immune response against Hepatitis B infection can be obtained through the administration of a single viral polypeptide, the Hepatitis B surface antigen (HBsAg). Thus, the Hepatitis B vaccine is generated through the utilization of recombinant DNA technology, preferentially by using yeast-based expression systems. However, the polypeptide needs to assemble into spherical particles, so-called virus-like particles (VLPs), to elicit the required protective immune response. So far, no clear evidence has been presented showing whether HBsAg assembles in vivo inside the yeast cell into VLPs or later in vitro during down-stream processing and purification.