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    The interaction of the gammaherpesvirus 68 orf73 protein with cellular BET proteins affects the activation of cell cycle promoters.

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    Authors
    Ottinger, Matthias
    Pliquet, Daniel
    Christalla, Thomas
    Frank, Ronald cc
    Stewart, James P
    Schulz, Thomas F
    Issue Date
    2009-05
    
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    Abstract
    Infection of mice with murine gammaherpesvirus 68 (MHV-68) provides a valuable animal model for gamma-2 herpesvirus (rhadinovirus) infection and pathogenesis. The MHV-68 orf73 protein has been shown to be required for the establishment of viral latency in vivo. This study describes a novel transcriptional activation function of the MHV-68 orf73 protein and identifies the cellular bromodomain containing BET proteins Brd2/RING3, Brd3/ORFX, and BRD4 as interaction partners for the MHV-68 orf73 protein. BET protein members are known to interact with acetylated histones, and Brd2 and Brd4 have been implicated in fundamental cellular processes, including cell cycle regulation and transcriptional regulation. Using MHV-68 orf73 peptide array assays, we identified Brd2 and Brd4 interaction sites in the orf73 protein. Mutation of one binding site led to a loss of the interaction with Brd2/4 but not the retinoblastoma protein Rb, to impaired chromatin association, and to a decreased ability to activate the BET-responsive cyclin D1, D2, and E promoters. The results therefore pinpoint the binding site for Brd2/4 in a rhadinoviral orf73 protein and suggest that the recruitment of a member of the BET protein family allows the MHV-68 orf73 protein to activate the promoters of G(1)/S cyclins. These findings point to parallels between the transcriptional activator functions of rhadinoviral orf73 proteins and papillomavirus E2 proteins.
    Citation
    The interaction of the gammaherpesvirus 68 orf73 protein with cellular BET proteins affects the activation of cell cycle promoters. 2009, 83 (9):4423-34 J. Virol.
    Affiliation
    Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
    Journal
    Journal of virology
    URI
    http://hdl.handle.net/10033/620745
    DOI
    10.1128/JVI.02274-08
    PubMed ID
    19244327
    Type
    Article
    Language
    en
    ISSN
    1098-5514
    ae974a485f413a2113503eed53cd6c53
    10.1128/JVI.02274-08
    Scopus Count
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    Publications of the research group Chemical Biology (CBIO)

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