14-3-3 proteins are constituents of the insoluble glycoprotein framework of the chlamydomonas cell wall.

Voigt, Jürgen; Frank, Ronald

dc.contributor.author	Voigt, Jürgen
dc.contributor.author	Frank, Ronald
dc.date.accessioned	2017-02-01T10:45:59Z
dc.date.available	2017-02-01T10:45:59Z
dc.date.issued	2003-06
dc.identifier.citation	14-3-3 proteins are constituents of the insoluble glycoprotein framework of the chlamydomonas cell wall. 2003, 15 (6):1399-413 Plant Cell	en
dc.identifier.issn	1040-4651
dc.identifier.pmid	12782732
dc.identifier.uri	http://hdl.handle.net/10033/620800
dc.description.abstract	The cell wall of the unicellular green alga Chlamydomonas reinhardtii consists predominantly of Hyp-rich glycoproteins, which also occur in the extracellular matrix of multicellular green algae and higher plants. In addition to the Hyp-rich polypeptides, the insoluble glycoprotein framework of the Chlamydomonas cell wall contains minor amounts of 14-3-3 proteins, as revealed by immunochemical studies and mass spectroscopic analysis of tryptic peptides. Polypeptides immunologically related to the 14-3-3 proteins also were found in the culture medium of Chlamydomonas. The levels of two of these 14-3-3-related polypeptides were decreased in the culture medium of the wall-deficient mutant cw-15. These findings indicate that 14-3-3 proteins are involved in the cross-linking of Hyp-rich glycoproteins in the Chlamydomonas cell wall.
dc.language.iso	en	en
dc.rights.uri	http://creativecommons.org/licenses/by-nc-sa/4.0/	*
dc.subject.mesh	14-3-3 Proteins	en
dc.subject.mesh	Algal Proteins	en
dc.subject.mesh	Amino Acid Sequence	en
dc.subject.mesh	Animals	en
dc.subject.mesh	Cell Wall	en
dc.subject.mesh	Chlamydomonas reinhardtii	en
dc.subject.mesh	Electrophoresis, Polyacrylamide Gel	en
dc.subject.mesh	Epitope Mapping	en
dc.subject.mesh	Glycoproteins	en
dc.subject.mesh	Glycosylation	en
dc.subject.mesh	Immunoblotting	en
dc.subject.mesh	Molecular Sequence Data	en
dc.subject.mesh	Protein Isoforms	en
dc.subject.mesh	Sequence Analysis, Protein	en
dc.subject.mesh	Tyrosine 3-Monooxygenase	en
dc.title	14-3-3 proteins are constituents of the insoluble glycoprotein framework of the chlamydomonas cell wall.	en
dc.type	Article	en
dc.contributor.department	Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.	en
dc.identifier.journal	The Plant cell	en
refterms.dateFOA	2018-06-12T17:53:43Z
html.description.abstract	The cell wall of the unicellular green alga Chlamydomonas reinhardtii consists predominantly of Hyp-rich glycoproteins, which also occur in the extracellular matrix of multicellular green algae and higher plants. In addition to the Hyp-rich polypeptides, the insoluble glycoprotein framework of the Chlamydomonas cell wall contains minor amounts of 14-3-3 proteins, as revealed by immunochemical studies and mass spectroscopic analysis of tryptic peptides. Polypeptides immunologically related to the 14-3-3 proteins also were found in the culture medium of Chlamydomonas. The levels of two of these 14-3-3-related polypeptides were decreased in the culture medium of the wall-deficient mutant cw-15. These findings indicate that 14-3-3 proteins are involved in the cross-linking of Hyp-rich glycoproteins in the Chlamydomonas cell wall.