The role of the poly(A) tract in the replication and virulence of tick-borne encephalitis virus.
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Authors
Asghar, NaveedLee, Yi-Ping
Nilsson, Emma
Lindqvist, Richard
Melik, Wessam
Kröger, Andrea
Överby, Anna K
Johansson, Magnus
Issue Date
2016-12-16
Metadata
Show full item recordAbstract
The tick-borne encephalitis virus (TBEV) is a flavivirus transmitted to humans, usually via tick bites. The virus causes tick-borne encephalitis (TBE) in humans, and symptoms range from mild flu-like symptoms to severe and long-lasting sequelae, including permanent brain damage. It has been suggested that within the population of viruses transmitted to the mammalian host, quasispecies with neurotropic properties might become dominant in the host resulting in neurological symptoms. We previously demonstrated the existence of TBEV variants with variable poly(A) tracts within a single blood-fed tick. To characterize the role of the poly(A) tract in TBEV replication and virulence, we generated infectious clones of Torö-2003 with the wild-type (A)3C(A)6 sequence (Torö-6A) or with a modified (A)3C(A)38 sequence (Torö-38A). Torö-38A replicated poorly compared to Torö-6A in cell culture, but Torö-38A was more virulent than Torö-6A in a mouse model of TBE. Next-generation sequencing of TBEV genomes after passaging in cell culture and/or mouse brain revealed mutations in specific genomic regions and the presence of quasispecies that might contribute to the observed differences in virulence. These data suggest a role for quasispecies development within the poly(A) tract as a virulence determinant for TBEV in mice.Citation
The role of the poly(A) tract in the replication and virulence of tick-borne encephalitis virus. 2016, 6:39265 Sci RepAffiliation
Helmholtz Centre for infection research. Inhoffenstr. 7. 38124 Braunschweig, Germany.Journal
Scientific reportsPubMed ID
27982069Type
ArticleLanguage
enISSN
2045-2322ae974a485f413a2113503eed53cd6c53
10.1038/srep39265
Scopus Count
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- Creative Commons
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-sa/4.0/