Browsing publications of the research group of microbial drugs (MWIS) by Authors
Phylogeny- and morphology-based recognition of new species in the spider-parasitic genus (Hypocreales, Cordycipitaceae) from Thailand.Kuephadungphan, Wilawan; Tasanathai, Kanoksri; Petcharad, Booppa; Khonsanit, Artit; Stadler, Marc; Luangsa-Ard, J Jennifer; HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany. (PenSoft publisher, 2020-09-02)Thailand is known to be a part of what is called the Indo-Burma biodiversity hotspot, hosting a vast array of organisms across its diverse ecosystems. This is reflected by the increasing number of new species described over time, especially fungi. However, a very few fungal species from the specialized spider-parasitic genus Gibellula have ever been reported from this region. A survey of invertebrate-pathogenic fungi in Thailand over several decades has led to the discovery of a number of fungal specimens with affinities to this genus. Integration of morphological traits into multi-locus phylogenetic analysis uncovered four new species: G. cebrennini, G. fusiformispora, G. pigmentosinum, and G. scorpioides. All these appear to be exclusively linked with torrubiella-like sexual morphs with the presence of granulomanus-like asexual morph in G. pigmentosinum and G. cebrennini. A remarkably high host specificity of these new species towards their spider hosts was revealed, and for the first time, evidence is presented for manipulation of host behavior in G. scorpioides.
Pigmentosins from Gibellula sp. As antibiofilm agents and a new glycosylated asperfuran from Cordyceps javanicaHelaly, Soleiman E.; Kuephadungphan, Wilawan; Phainuphong, Patima; Ibrahim, Mahmoud A.A.; Tasanathai, Kanoksri; Mongkolsamrit, Suchada; Luangsa-Ard, Janet Jennifer; Phongpaichit, Souwalak; Rukachaisirikul, Vatcharin; Stadler, Marc; et al. (Beilstein Institut, 2019-12-16)n the course of our exploration of the Thai invertebrate-pathogenic fungi for biologically active metabolites, pigmentosin A (1) and a new bis(naphtho-α-pyrone) derivative, pigmentosin B (2), were isolated from the spider-associated fungus Gibellula sp. Furthermore, a new glycosylated asperfuran 3, together with one new (6) and two known (4 and 5) cyclodepsipeptides, was isolated from Cordyceps javanica. The pigmentosins 1 and 2 showed to be active against biofilm formation of Staphylococcus aureus DSM1104. The lack of toxicity toward the studied microorganism and cell lines of pigmentosin B (2), as well as the antimicrobial effect of pigmentosin A (1), made them good candidates for further development for use in combination therapy of infections involving biofilm-forming S. aureus. The structure elucidation and determination of the absolute configuration were accomplished using a combination of spectroscopy, including 1D and 2D NMR, HRMS, Mosher ester analysis, and comparison of calculated/experimental ECD spectra. A chemotaxonomic investigation of the secondary metabolite profiles using analytical HPLC coupled with diode array detection and mass spectrometry (HPLC–DAD–MS) revealed that the production of pigmentosin B (2) was apparently specific for Gibellula sp., while the glycoasperfuran 3 was specific for C. javanica.
Studies on the biologically active secondary metabolites of the new spider parasitic fungus Gibellula gamsiiKuephadungphan, Wilawan; Macabeo, Allan Patrick G.; Luangsa-Ard, Janet Jennifer; Tasanathai, Kanoksri; Thanakitpipattana, Donnaya; Phongpaichit, Souwalak; Yuyama, Kamila; Stadler, Marc