• Haprolid Inhibits Tumor Growth of Hepatocellular Carcinoma through Rb/E2F and Akt/mTOR Inhibition.

      Xing, Jun; Bhuria, Vikas; Bui, Khac Cuong; Nguyen, Mai Ly Thi; Hu, Zexi; Hsieh, Chih-Jen; Wittstein, Kathrin; Stadler, Marc; Wilkens, Ludwig; Li, Jun; et al. (MDPI, 2020-03-06)
      The efficacy of haprolid was evaluated in human HCC cell lines (Huh-7, Hep3B and HepG2) and xenograft tumors (NMRI-Foxn1nu mice with injection of Hep3B cells). Cytotoxic activity of haprolid was determined by the WST-1 and crystal violet assay. Wound healing, transwell and tumorsphere assays were performed to investigate migration and invasion of HCC cells. Apoptosis and cell-cycle distribution were measured by flow cytometry. The effects of haprolid on the Rb/E2F and Akt/mTOR pathway were examined by immunoblotting and immunohistochemistry.