ImmunoPET/MR imaging allows specific detection of Aspergillus fumigatus lung infection in vivo.
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Authors
Rolle, Anna-MariaHasenberg, Mike
Thornton, Christopher R
Solouk-Saran, Djamschid
Männ, Linda
Weski, Juliane
Maurer, Andreas
Fischer, Eliane
Spycher, Philipp R
Schibli, Roger
Boschetti, Frederic
Stegemann-Koniszewski, Sabine
Bruder, Dunja
Severin, Gregory W
Autenrieth, Stella E
Krappmann, Sven
Davies, Genna
Pichler, Bernd J
Gunzer, Matthias
Wiehr, Stefan
Issue Date
2016-02-23
Metadata
Show full item recordAbstract
Invasive pulmonary aspergillosis (IPA) is a life-threatening lung disease caused by the fungus Aspergillus fumigatus, and is a leading cause of invasive fungal infection-related mortality and morbidity in patients with hematological malignancies and bone marrow transplants. We developed and tested a novel probe for noninvasive detection of A. fumigatus lung infection based on antibody-guided positron emission tomography and magnetic resonance (immunoPET/MR) imaging. Administration of a [(64)Cu]DOTA-labeled A. fumigatus-specific monoclonal antibody (mAb), JF5, to neutrophil-depleted A. fumigatus-infected mice allowed specific localization of lung infection when combined with PET. Optical imaging with a fluorochrome-labeled version of the mAb showed colocalization with invasive hyphae. The mAb-based newly developed PET tracer [(64)Cu]DOTA-JF5 distinguished IPA from bacterial lung infections and, in contrast to [(18)F]FDG-PET, discriminated IPA from a general increase in metabolic activity associated with lung inflammation. To our knowledge, this is the first time that antibody-guided in vivo imaging has been used for noninvasive diagnosis of a fungal lung disease (IPA) of humans, an approach with enormous potential for diagnosis of infectious diseases and with potential for clinical translation.Citation
ImmunoPET/MR imaging allows specific detection of Aspergillus fumigatus lung infection in vivo. 2016, 113 (8):E1026-33 Proc. Natl. Acad. Sci. U.S.A.Affiliation
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.PubMed ID
26787852Type
ArticleLanguage
enISSN
1091-6490ae974a485f413a2113503eed53cd6c53
10.1073/pnas.1518836113
Scopus Count
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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-sa/4.0/
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