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dc.contributor.authorRolle, Anna-Maria
dc.contributor.authorHasenberg, Mike
dc.contributor.authorThornton, Christopher R
dc.contributor.authorSolouk-Saran, Djamschid
dc.contributor.authorMänn, Linda
dc.contributor.authorWeski, Juliane
dc.contributor.authorMaurer, Andreas
dc.contributor.authorFischer, Eliane
dc.contributor.authorSpycher, Philipp R
dc.contributor.authorSchibli, Roger
dc.contributor.authorBoschetti, Frederic
dc.contributor.authorStegemann-Koniszewski, Sabine
dc.contributor.authorBruder, Dunja
dc.contributor.authorSeverin, Gregory W
dc.contributor.authorAutenrieth, Stella E
dc.contributor.authorKrappmann, Sven
dc.contributor.authorDavies, Genna
dc.contributor.authorPichler, Bernd J
dc.contributor.authorGunzer, Matthias
dc.contributor.authorWiehr, Stefan
dc.date.accessioned2017-03-16T09:40:13Z
dc.date.available2017-03-16T09:40:13Z
dc.date.issued2016-02-23
dc.identifier.citationImmunoPET/MR imaging allows specific detection of Aspergillus fumigatus lung infection in vivo. 2016, 113 (8):E1026-33 Proc. Natl. Acad. Sci. U.S.A.en
dc.identifier.issn1091-6490
dc.identifier.pmid26787852
dc.identifier.doi10.1073/pnas.1518836113
dc.identifier.urihttp://hdl.handle.net/10033/620863
dc.description.abstractInvasive pulmonary aspergillosis (IPA) is a life-threatening lung disease caused by the fungus Aspergillus fumigatus, and is a leading cause of invasive fungal infection-related mortality and morbidity in patients with hematological malignancies and bone marrow transplants. We developed and tested a novel probe for noninvasive detection of A. fumigatus lung infection based on antibody-guided positron emission tomography and magnetic resonance (immunoPET/MR) imaging. Administration of a [(64)Cu]DOTA-labeled A. fumigatus-specific monoclonal antibody (mAb), JF5, to neutrophil-depleted A. fumigatus-infected mice allowed specific localization of lung infection when combined with PET. Optical imaging with a fluorochrome-labeled version of the mAb showed colocalization with invasive hyphae. The mAb-based newly developed PET tracer [(64)Cu]DOTA-JF5 distinguished IPA from bacterial lung infections and, in contrast to [(18)F]FDG-PET, discriminated IPA from a general increase in metabolic activity associated with lung inflammation. To our knowledge, this is the first time that antibody-guided in vivo imaging has been used for noninvasive diagnosis of a fungal lung disease (IPA) of humans, an approach with enormous potential for diagnosis of infectious diseases and with potential for clinical translation.
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subject.meshAnimalsen
dc.subject.meshAntibodies, Fungalen
dc.subject.meshAntibodies, Monoclonal, Murine-Deriveden
dc.subject.meshAspergillus fumigatusen
dc.subject.meshHumansen
dc.subject.meshMagnetic Resonance Imagingen
dc.subject.meshMiceen
dc.subject.meshPositron-Emission Tomographyen
dc.subject.meshPulmonary Aspergillosisen
dc.subject.meshRadiographyen
dc.titleImmunoPET/MR imaging allows specific detection of Aspergillus fumigatus lung infection in vivo.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalProceedings of the National Academy of Sciences of the United States of Americaen
refterms.dateFOA2018-06-13T09:08:04Z
html.description.abstractInvasive pulmonary aspergillosis (IPA) is a life-threatening lung disease caused by the fungus Aspergillus fumigatus, and is a leading cause of invasive fungal infection-related mortality and morbidity in patients with hematological malignancies and bone marrow transplants. We developed and tested a novel probe for noninvasive detection of A. fumigatus lung infection based on antibody-guided positron emission tomography and magnetic resonance (immunoPET/MR) imaging. Administration of a [(64)Cu]DOTA-labeled A. fumigatus-specific monoclonal antibody (mAb), JF5, to neutrophil-depleted A. fumigatus-infected mice allowed specific localization of lung infection when combined with PET. Optical imaging with a fluorochrome-labeled version of the mAb showed colocalization with invasive hyphae. The mAb-based newly developed PET tracer [(64)Cu]DOTA-JF5 distinguished IPA from bacterial lung infections and, in contrast to [(18)F]FDG-PET, discriminated IPA from a general increase in metabolic activity associated with lung inflammation. To our knowledge, this is the first time that antibody-guided in vivo imaging has been used for noninvasive diagnosis of a fungal lung disease (IPA) of humans, an approach with enormous potential for diagnosis of infectious diseases and with potential for clinical translation.


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