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dc.contributor.authorPereira, Rui P A
dc.contributor.authorPeplies, Jörg
dc.contributor.authorBrettar, Ingrid
dc.contributor.authorHöfle, Manfred G
dc.date.accessioned2017-04-07T08:45:33Z
dc.date.available2017-04-07T08:45:33Z
dc.date.issued2017-03-31
dc.identifier.citationDevelopment of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems. 2017, 17 (1):79 BMC Microbiol.en
dc.identifier.issn1471-2180
dc.identifier.pmid28359254
dc.identifier.doi10.1186/s12866-017-0987-5
dc.identifier.urihttp://hdl.handle.net/10033/620890
dc.description.abstractNext Generation Sequencing (NGS) has revolutionized the analysis of natural and man-made microbial communities by using universal primers for bacteria in a PCR based approach targeting the 16S rRNA gene. In our study we narrowed primer specificity to a single, monophyletic genus because for many questions in microbiology only a specific part of the whole microbiome is of interest. We have chosen the genus Legionella, comprising more than 20 pathogenic species, due to its high relevance for water-based respiratory infections.
dc.language.isoenen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleDevelopment of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems.en
dc.typeArticleen
dc.contributor.departmentHelmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.en
dc.identifier.journalBMC microbiologyen
refterms.dateFOA2018-06-12T16:48:13Z
html.description.abstractNext Generation Sequencing (NGS) has revolutionized the analysis of natural and man-made microbial communities by using universal primers for bacteria in a PCR based approach targeting the 16S rRNA gene. In our study we narrowed primer specificity to a single, monophyletic genus because for many questions in microbiology only a specific part of the whole microbiome is of interest. We have chosen the genus Legionella, comprising more than 20 pathogenic species, due to its high relevance for water-based respiratory infections.


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